PE Rabbit anti-Human CD81 Monoclonal Antibody for Flow Cytometry - P60033

Core Product Specifications and Parameters

Biological Background: CD81 Function and Localization

• CD81 is a 26 kDa tetraspanin, also known as TAPA-1 or TSPAN28, belonging to the tetraspanin family of cell surface proteins that organize signaling microdomains.
• In B cells, CD81 facilitates the assembly of CD19 and B cell receptor complexes at tetraspanin-enriched microdomains, lowering the antigen threshold required for clonal expansion and antibody production. Related references: PMID:15161911, PMID:20237408
• In T cells, CD81 localizes CD3 zeta at immunological synapses with B cells, promoting costimulation and T helper type 2 polarization. Related references: PMID:22307619, PMID:23858057, PMID:8766544
• CD81 localizes to the cell membrane and basolateral membrane, and is also present in MHC class II compartments, suggesting a role in antigen presentation. Related references: PMID:8409388, PMID:8766544
• CD81 negatively regulates cell fusion: it inhibits macrophage fusion into multinucleated giant cells, and also restricts myotube and osteoclast fusion. Related references: PMID:12796480
• Acts as a receptor for hepatitis C virus (HCV) entry into hepatocytes by associating with CLDN1, and is also involved in HIV-1 replication restriction via SAMHD1 degradation.
• Expressed on B cells, hepatocytes, monocytes/macrophages, and both naive and memory CD4+ T cells.

Experimental Guidance and Technical Tips

• Titrate the PE-conjugated antibody to determine the optimal staining concentration for your flow cytometry assay.
• CD81 is a cell surface antigen; surface staining can be performed without permeabilization. Validate staining in relevant human cell types such as B cells, T cells, or cell lines expressing CD81.
• The immunogen corresponds to the large extracellular loop (amino acids 114-199), a region critical for receptor interactions and exposed on the cell surface.
• As a monoclonal antibody, this reagent offers consistent lot-to-lot performance. Consider using appropriate isotype controls and viability dyes to ensure accurate gating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering a wide range of antibodies, antigen proteins, and ancillary reagents for diagnostic development. Our portfolio includes validated flow cytometry antibodies like this PE anti-CD81 clone, supporting immunology and infectious disease research. Trust CamelBio to bridge your laboratory needs with reliable supply chains from concept to clinic.