PE Rabbit anti-Human CD80/B7-1 Monoclonal Antibody for FC - P33681

Core Product Specifications and Parameters

Biological Background: CD80/B7-1 Function and Localization

• CD80 (B7-1) is an immunoglobulin superfamily costimulatory molecule essential for T-lymphocyte activation, providing the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells.
  Related references:
  PMID:12196291

• It acts as a ligand for the CD28 receptor, initiating downstream signaling cascades including NF-kappa-B and MAPK pathways, which promote cytokine production and T-cell proliferation.
  Related references:
  PMID:10438913

• As a CTLA-4 counter-receptor, CD80 delivers inhibitory signals that downregulate T-cell responses by blocking CD28-mediated co-stimulation and maintaining immune homeostasis.
  Related references:
  PMID:10583602
  PMID:11279502

• CD28/CD80 engagement enhances glucose metabolism and ATP synthesis through the PI3K/Akt pathway, supporting the energetic demands of activated T-cells.
  Related references:
  PMID:12121659

• CD80 contributes to PD-L1/PD-1 pathway regulation by limiting excessive PD-L1 engagement, thus balancing immune activation and tolerance.
  Related references:
  PMID:36727298

• Expressed on antigen-presenting cells (APCs), CD80 plays a critical role in B-cell/T-cell interactions within germinal centers, driving effective humoral immunity.

• The protein is a single-pass type I membrane glycoprotein localized to the cell membrane and undergoes post-translational modifications including palmitoylation and phosphorylation.

• (Microbial infection) Serves as a receptor for adenovirus subgroup B.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody targets the extracellular domain of human CD80 (immunogen aa 35-242), making it suitable for direct flow cytometry staining of cell surface antigen on viable cells.
• Verify optimal working concentration by titration using a positive control sample (e.g., activated B-cells or CD80-transfected cell lines).
• Include appropriate isotype control and Fc receptor blocking steps to minimize non-specific binding.
• For multicolor flow cytometry panels, consider spectral overlap compensation when using PE.
• Store protected from light at 2–8°C and avoid repeated freeze-thaw cycles to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic one-stop partner for IVD raw-material procurement, bridging the gap from concept to clinic. This anti-CD80 monoclonal antibody supports immuno-oncology research and diagnostic development. Beyond validated antibodies, CamelBio supplies bulk ancillary reagents, optimized antibody pairs, and can source rare-target raw materials to accelerate your assay development and manufacturing.