PE Rabbit anti-Human CD79b/Igβ Monoclonal Antibody for FC - P40259

Core Product Specifications and Parameters

Biological Background: CD79b/Igβ Function and Localization

• CD79b (Ig-beta) is the beta chain of the B-cell antigen receptor complex, required for B-cell development and function.
• In cooperation with CD79A, it initiates signal transduction upon BCR activation, leading to complex internalization, trafficking to late endosomes, and antigen presentation.
• CD79b enhances phosphorylation of CD79A, likely by recruiting kinases or protecting it from dephosphorylation, thereby amplifying the signaling cascade.
• The protein contains an immunoglobulin domain, a transmembrane helix, and an ITAM-containing cytoplasmic tail crucial for downstream effector activation.
• Subcellular localization is the cell membrane; expression is tightly restricted to B-cells, as indicated by tissue specificity data.
• Post-translational modifications include glycosylation, phosphorylation, disulfide bond formation, and ubiquitination, which modulate receptor stability and signaling.
• Alternative splicing produces isoforms; mutations in CD79B are associated with primary immunodeficiencies (disease variant keyword).

Experimental Guidance and Technical Tips

• For flow cytometry, use PE-compatible lasers and detectors; titrate the antibody to determine the optimal staining concentration for your sample type.
• The immunogen (amino acids 29-159) targets the extracellular domain; thus the antibody is expected to recognize native surface CD79b on viable B-cells.
• Validate specificity by including appropriate isotype controls and using B-cell lines (positive) versus T-cell or non-hematopoietic lines (negative).
• CD79b expression may be influenced by B-cell activation status; include both resting and activated controls in experiments.
• The observed protein weight range (14 kDa/26 kDa) reflects glycosylation differences; if applying in western blotting, note that band size may vary.
• Ensure sample processing maintains cell surface integrity; avoid harsh fixation that could damage epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop procurement solution for IVD raw materials, connecting diagnostic manufacturers and labs with high-quality reagents like this CD79b antibody. Our platform streamlines access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents, complementing your B-cell research and diagnostic panel development. Leverage our capability to source rare-target raw materials and scale your project from concept to clinic efficiently.