PE Rabbit anti-Human CD72 Monoclonal Antibody for Flow Cytometry - P21854

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• CD72, also known as Lyb-2, is a B-cell differentiation antigen encoded by the CD72 gene in humans.
• Functions as a co-receptor of the B cell receptor (BCR), playing both positive and negative regulatory roles in B-cell functions.
• Recognizes the Sm/ribonucleoprotein (RNP) self-antigen; coligation of CD72 with BCR inhibits BCR signaling by recruiting PTPN6/SHP-1 to the BCR complex.
• Acts as a ligand for CD5, playing a critical role in maintaining regulatory T and B-cell homeostasis.
• Localizes to the cell membrane as a single-pass type II membrane protein with a signal-anchor.
• Contains a C-type lectin domain and is post-translationally modified by disulfide bonds and phosphorylation.
• Expressed specifically in pre-B-cells and B-cells, but not in terminally differentiated plasma cells.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is designed for direct flow cytometry; no secondary antibody is required.
• The immunogen covers the extracellular region (amino acids 117-359), ensuring binding to the native cell surface form of CD72.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and live/dead discrimination to ensure accurate gating of B-cell populations.
• Consider using B-cell lines (e.g., Raji, Daudi) or primary PBMCs for validation and routine assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a wide range of IVD raw materials, including validated antibodies, antibody pairs, and bulk ancillary reagents. For targets like CD72, which play a vital role in B-cell biology and immune regulation, we offer tailored procurement solutions—from monoclonal/polyclonal antibodies to rare target sourcing—backed by technical consultation throughout your development pipeline. Contact us to explore how our integrated supply chain can support your next diagnostic project.