PE Rabbit anti-Human CD7 Monoclonal Antibody for Flow Cytometry - P09564

Core Product Specifications and Parameters

Biological Background: CD7 Function and Localization

• CD7 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, also designated as GP40, TP41, Tp40, and Leu-9.
• It is primarily expressed on T-cells and natural killer (NK) cells and their precursors, serving as a costimulatory molecule during T-cell activation through interaction with its ligand K12/SECTM1. Related references: PMID:10652336
• CD7 engagement triggers intracellular signaling that leads to the production of cytokines, including interleukin-2 (IL-2) in T-cells and interferon-gamma in resting NK cells. Related references: PMID:1709867 PMID:7506726
• The protein localizes to the plasma membrane via a single-pass transmembrane helix and contains an extracellular immunoglobulin domain.
• Post-translational modifications include disulfide bond formation, N‑glycosylation, and palmitoylation, the latter contributing to membrane raft association.
• CD7 is a well-established marker for immunophenotyping of normal and malignant T/NK-cell populations and is implicated in leukemic diagnostics.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 26‑180) corresponds to the extracellular domain; the antibody is expected to recognize native surface CD7 on live cells, making it suitable for flow cytometry without permeabilization.
• For flow cytometry, use human peripheral blood mononuclear cells (PBMCs) or purified T‑cell / NK‑cell populations. Perform antibody titration (e.g., 5 μL per 1×10⁶ cells) and include appropriate isotype control.
• Avoid light exposure during staining and storage to preserve PE fluorescence. Keep the antibody on ice during use as specified.
• Validate the staining pattern by comparing with established CD7 clones; consider co‑staining with CD3 or CD56 markers to confirm lineage specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement for diagnostic developers, offering a comprehensive portfolio of validated antibodies, including this PE‑conjugated anti‑CD7 monoclonal antibody. For flow cytometry‑based diagnostics and T‑cell/NK‑cell research, we can supply matched antibody pairs, bulk reagents, and rare‑target sourcing to accelerate your assay development. Partner with CamelBio to bridge the gap from concept to clinical application efficiently.