PE Rabbit anti-Human CD63 Monoclonal Antibody for FC - P08962

Core Product Specifications and Parameters

Biological Background: CD63 Function and Localization

• CD63, also known as granulophysin, LAMP-3, ME491, and Tspan-30, is a member of the tetraspanin superfamily encoded by the CD63 gene, with alternative splicing producing multiple isoforms.
• It functions as a cell surface receptor for TIMP1, activating downstream signaling cascades involving AKT, FAK, and MAP kinases, which promote cell survival, adhesion, and migration.
• CD63 plays a key role in integrin signaling by facilitating ITGB1 activation, leading to actin cytoskeleton reorganization and enhanced cell spreading.
• In VEGFA signaling, CD63 regulates the internalization of KDR/VEGFR2, influencing angiogenesis-related pathways.
• The protein is essential for intracellular trafficking, particularly for the PMEL luminal domain, which is critical for melanosome maturation and melanocyte development.
• Subcellularly, CD63 localizes to the plasma membrane, lysosomal and late endosomal membranes, multivesicular bodies, melanosomes, and is also secreted via extracellular exosomes.
• Tissue expression includes high levels in platelets (protein level), dysplastic nevi, primary melanomas, hematopoietic cells, and tissue macrophages.
• Post-translational modifications, such as N-linked glycosylation and palmitoylation, are important for its membrane organization and functional interactions.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 1–238) of human CD63; this design may be suitable for detecting surface‑expressed CD63 by flow cytometry.
• For FC applications, include appropriate isotype controls and perform antibody titration to optimize the signal‑to‑noise ratio.
• Maintain samples under conditions that preserve cell surface protein integrity to ensure accurate detection of CD63.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, empowering your CD63‑focused studies in cancer, immunology, and melanoma research. As a one‑stop sourcing partner, we connect diagnostic manufacturers and laboratories with the critical components needed from early development through clinical application.