PE Rabbit anti-Human CD62L/L-Selectin Monoclonal Antibody for FC - P14151

Core Product Specifications and Parameters

Biological Background: CD62L/L-Selectin Function and Localization

• L-selectin (SELL), also known as CD62L, LAM-1, LECAM1, Leu-8, TQ1, and lymph node homing receptor, is a member of the selectin family of adhesion molecules.
• It functions as a calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells, promoting initial tethering and rolling of leukocytes on endothelium. Related references: PMID:12403782 PMID:28011641 PMID:28489325
• The protein is localized to the cell membrane.
• Expressed primarily in B-cell lines and T-lymphocytes.
• Characterized by multiple structural domains including a C-type lectin domain, an EGF-like domain, and Sushi (CCP/SCR) domains; it is a glycoprotein with disulfide bonds and transmembrane regions, and its activity depends on calcium binding.

Experimental Guidance and Technical Tips

• This PE-conjugated rabbit monoclonal antibody is designed for flow cytometry (FC) applications.
• The immunogen encompasses the extracellular region (amino acids 39-332), suggesting the antibody is likely to recognize the native surface conformation of L-selectin.
• For FC, consider titrating the antibody to determine the optimal concentration for your specific experimental setup, and include appropriate isotype control and viability staining.
• Validate the staining pattern on known L-selectin-positive human lymphocytes (e.g., T cells or B cells) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio that includes validated monoclonal antibodies like this anti-CD62L/L-Selectin clone, as well as ancillary reagents and custom sourcing solutions. Whether you need bulk quantities, matched antibody pairs, or rare-target reagents, our B2B platform streamlines procurement for diagnostic developers and researchers.