PE Rabbit anti-Human CD62E/E-Selectin mAb for FC - P16581

Core Product Specifications and Parameters

Biological Background: E-selectin Function and Localization

• E-selectin, also known as CD62E, endothelial leukocyte adhesion molecule 1 (ELAM-1), and leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a cell-surface glycoprotein encoded by the SELE gene (GeneID: 6401).
• It mediates the adhesion of blood neutrophils to cytokine-activated endothelium through interaction with SELPLG/PSGL1, playing a central role in immunoadhesion and leukocyte recruitment during inflammation.
• The protein contains an N-terminal C-type lectin domain, an EGF-like domain, and multiple short consensus repeat (sushi) domains, characteristic of selectins, and requires calcium for function.
• It is a transmembrane protein localized at the cell membrane, with disulfide bonds stabilizing its extracellular domains and a single transmembrane helix anchoring it to the surface.
• E-selectin may also contribute to capillary morphogenesis, suggesting broader roles in vascular biology beyond immune cell trafficking.
• The mature protein has an approximate molecular weight of 66 kDa (UniProt: P16581), consistent with the product specification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22–230 of human E-selectin, encompassing the extracellular lectin and EGF-like domains, which are critical for ligand binding; this design supports specificity for the native receptor.
• For flow cytometry, consider using activated human endothelial cells (e.g., HUVECs stimulated with TNF-α or IL-1β) as a positive control, and validate binding specificity with appropriate blocking peptides or isotype controls.
• The PE conjugate allows direct single-step staining, minimizing background and procedure time; titrate the antibody to optimize signal-to-noise ratio for your cell system.
• Confirm expression of the 66 kDa target in your sample by referencing the SWISS-PROT entry P16581, and consider performing a molecular weight verification if switching to western blot assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, including validated monoclonal/polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents for diverse targets like CD62E relevant to inflammation, cardiovascular, and immuno-oncology research. This antibody exemplifies our capability to supply rare-target raw materials with detailed characterization, helping you accelerate assay development from concept to clinic. For your E-selectin projects, we can source the complete workflow—from primary antibodies to detection reagents—ensuring reliability and scalability for your diagnostic or research needs.