PE Rabbit anti-Human CD61 Monoclonal Antibody for FC - P05106

Core Product Specifications and Parameters

Biological Background: Integrin beta-3 (CD61) Function and Localization

• Integrin beta-3 (CD61, GPIIIa) forms heterodimers with integrin alpha-V or alpha-IIb to create receptors for a broad range of ligands, including fibronectin, fibrinogen, vitronectin, von Willebrand factor, and osteopontin. These interactions often recognize the RGD tripeptide motif.
• Upon activation, ITGA2B:ITGB3 mediates platelet aggregation by binding soluble fibrinogen, a critical step in hemostasis. Related references: PMID:9111081
• ITGAV:ITGB3 acts as a coreceptor for CX3CL1 (fractalkine), facilitating CX3CR1-dependent signaling, and serves as a receptor for various growth factors including FGF1, FGF2, IGF1, IGF2, IL1B, and NRG1, essential for their downstream signaling. Related references: PMID:23125415 PMID:24789099 PMID:20682778 PMID:18441324 PMID:28302677 PMID:19578119 PMID:28873464 PMID:29030430
• Integrin beta-3 is localized at the cell membrane, lamellipodium membrane, focal adhesions, and postsynaptic membrane, consistent with its roles in cell adhesion, migration, and synaptic function.
• In the brain, ITGB3 is involved in synaptic transmission and plasticity, regulating serotonin reuptake by controlling SLC6A4 localization.
• Isoforms beta-3A and beta-3C are produced by alternative splicing; beta-3A is specifically expressed in osteoblasts, while beta-3C is specific to prostate and testis.
• ITGAV:ITGB3 also functions as a receptor for several viruses, including Herpes virus 8, Coxsackievirus A9, Hantaan virus, and Cytomegalovirus, facilitating host cell entry.
• Key structural features include EGF-like domains, disulfide bonds, and multiple phosphorylation sites. Mutations in ITGB3 are associated with Glanzmann thrombasthenia and platelet-type bleeding disorders.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal working concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and viability dyes to ensure specific staining and accurate gating.
• Protect the PE conjugate from prolonged exposure to light to maintain fluorescence intensity.
• Prepare fresh, single-cell suspensions to avoid aggregation and ensure consistent staining.
• The immunogen corresponds to the extracellular domain (aa 21-718), so this antibody is suitable for surface staining of live, unfixed cells. Validate staining in your relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-CD61 antibody targets integrin beta-3, a key player in platelet biology and hemostasis, making it valuable for thrombosis and cardiovascular disease research. CamelBio’s supply portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials to support your diagnostic development needs.