PE Rabbit anti-Human CD6 Monoclonal Antibody for Flow Cytometry - P30203

Core Product Specifications and Parameters

Biological Background: CD6 Function and Localization

• CD6 (T-cell differentiation antigen CD6, also known as T12 or TP120) is a type I transmembrane glycoprotein expressed primarily on T cells, NK cells, and a subset of B cells (B-1 cells).
• CD6 functions as a cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses through interaction with ALCAM/CD166.
  Related references: PMID:15048703, PMID:15294938, PMID:16352806
• It contributes to signaling cascades triggered by TCR/CD3 complex activation and acts as a costimulatory molecule, promoting T-cell activation and proliferation.
  Related references: PMID:15294938, PMID:16352806, PMID:16914752
• CD6 is involved in the formation and maturation of the immunological synapse.
  Related references: PMID:15294938, PMID:16352806
• CD6 also functions as a calcium-dependent pattern recognition receptor that binds both Gram-positive and Gram-negative bacteria via LPS and lipoteichoic acid, leading to MAP kinase activation and pro-inflammatory cytokine secretion.
  Related references: PMID:17601777
• It is localized to the cell membrane and can be shed as a soluble form detected in serum, suggesting roles in both membrane-proximal and systemic immune regulation.
• Tissue expression profile: high in thymus, spleen, lymph node, appendix, and peripheral blood leukocytes; detected on thymocytes, mature T cells, NK cells, B-1 cells, and some brain cells.
• Alternative splicing produces multiple isoforms with molecular weights ranging from 58 to 71 kDa; the protein undergoes N-glycosylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 18-364 of human CD6) covers the extracellular region containing the ligand-binding domains; this design may favor recognition of native surface CD6 in flow cytometry.
• For flow cytometry, titrate the antibody to determine optimal concentration for your specific cell type; include appropriate isotype control antibodies.
• CD6 is shed from the cell surface; the presence of soluble CD6 in serum or culture supernatants could potentially compete with membrane-bound CD6 staining; consider this in experimental design.
• When analyzing T-cell subsets, note that CD6 expression levels may vary with activation state; validate staining patterns in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic supply partner for IVD manufacturers and research laboratories, offering a comprehensive portfolio of raw materials from early concept to clinical application. This anti-CD6 monoclonal antibody supports immunophenotyping and T-cell research applications. We provide validated antibody pairs, bulk monoclonals, and custom sourcing for rare immunological targets to help you streamline assay development and procurement.