PE Rabbit anti-Human CD56/NCAM1 Monoclonal Antibody for FC - P13591

Core Product Specifications and Parameters

Biological Background: NCAM1 Function and Localization

• Encodes neural cell adhesion molecule 1 (NCAM1), a member of the immunoglobulin superfamily involved in homophilic and heterophilic cell-cell adhesion.
• Plays a central role in neuron-neuron adhesion, neurite fasciculation, and promotion of neurite outgrowth during nervous system development and plasticity.
• Acts as a host cell receptor for rabies virus and Zika virus, mediating viral entry into cells.
• Localizes predominantly to the cell membrane, with multiple isoforms arising from alternative splicing, including transmembrane and GPI-anchored forms; secreted isoforms have also been reported.
• Contains extracellular immunoglobulin-like domains and fibronectin type III repeats, facilitating interactions with other NCAM molecules and extracellular matrix components.
• Post-translationally modified by N-linked glycosylation and phosphorylation, which modulate its adhesive properties and intracellular signaling.
• Expressed broadly in neural tissues, with additional expression in natural killer cells, subsets of T lymphocytes, and various tumors, serving as a clinical marker (CD56) in immunophenotyping.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 20–413), suggesting the antibody likely recognizes an epitope accessible on live cells for flow cytometry.
• For flow cytometry, titrate the PE conjugate to determine optimal staining concentration and include appropriate Fc receptor blocking to reduce nonspecific binding.
• Validate the antibody in human samples known to express CD56, such as NK cell lines, primary NK cells, or neuronal cell lines, and use isotype controls to confirm specificity.
• If studying viral receptor function, consider using the antibody in competitive binding assays or to monitor NCAM1 expression modulation upon pathogen challenge.
• Note that due to alternative splicing, some NCAM1 isoforms may not be detected if the epitope is masked or absent; confirm with appropriate recombinant controls if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering a reliable supply chain for validated monoclonal antibodies, ancillary reagents, and rare target procurables. Our product portfolio, including this PE-conjugated anti-CD56 antibody, addresses key research areas such as neurobiology, immunology, and virology. Whether you need bulk anti-NCAM antibodies for diagnostic kit development or specialized reagents for viral entry studies, CamelBio bridges the gap from concept to clinic with technical and supply chain support.