PE Rabbit anti-Human CD55 Monoclonal Antibody for FC - P08174

Core Product Specifications and Parameters

Biological Background: CD55 (DAF) Function and Localization

• CD55 (complement decay-accelerating factor), also known as DAF, CR, TC, CROM, or CHAPLE, is a GPI-anchored membrane glycoprotein that plays a critical role in regulating the complement system.
• CD55 recognizes C4b and C3b fragments, interfering with their ability to catalyze the formation of C4b2a and C3bBb convertases (PubMed:7525274). It inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, thereby protecting host cells from complement damage (PubMed:28657829). Related references: PMID:7525274 PMID:28657829
• It localizes primarily to the cell membrane via a GPI anchor but is also found in secreted forms in body fluids and extracellular matrix.
• CD55 is expressed on the plasma membranes of all cell types in intimate contact with plasma complement proteins and on epithelial cells lining extracellular compartments, indicating broad tissue distribution.
• CD55 acts as a receptor for multiple viruses, including Coxsackievirus A21, B1, B3, B5; likely Human enterovirus 70 and D68; and echoviruses 6, 7, 11, 12, 20, and 21, linking it to host-virus interactions.
• Mutations in CD55 are associated with CHAPLE syndrome, a disease characterized by complement hyperactivation, angiopathic thrombosis, and protein-losing enteropathy, underscoring its essential role in innate immunity.

Experimental Guidance and Technical Tips

• PE direct conjugation enables detection in flow cytometry without a secondary antibody; titrate the antibody to determine the optimal staining concentration for your specific cell type.
• The immunogen spans amino acids 35-353, covering the extracellular domain of human CD55; verify reactivity if using cells from alternative species or engineered models.
• CD55 is broadly expressed; include appropriate isotype controls and fluorescence compensation controls to ensure signal specificity.
• Consider combining with a viability dye or a dump channel when detecting CD55 in heterogeneous cell populations, as some myeloid cells may express the target at varying levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. Our portfolio, including this PE anti-human CD55 antibody, addresses complement biology, immunology, and virology research needs. Beyond single reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development and production.