PE Rabbit anti-Human CD52 Monoclonal Antibody for Flow Cytometry - P31358

Core Product Specifications and Parameters

Biological Background: CD52 Function and Localization

• CD52, also known as CAMPATH-1 antigen, HE5, CDW52, and EDDM5, is a GPI-anchored glycoprotein predominantly found on the cell membrane.
• The protein is expressed as a small, mature peptide with a molecular weight of approximately 7 kDa, anchored to the lipid bilayer via a glycosylphosphatidylinositol (GPI) moiety.
• Functional studies suggest CD52 may play a role in carbohydrate presentation and orientation, although its precise biological function remains to be fully elucidated.
• CD52 was originally identified as the Cambridge pathology 1 antigen and is also recognized as an epididymal secretory protein (HE5), indicating tissue-specific roles in the epididymis.
• The extracellular N-terminal region, which includes the peptide immunogen used, is accessible for antibody binding, making CD52 a suitable target for flow cytometric detection on live cells.
• Post-translational modifications include N-linked glycosylation, and the protein is attached to the membrane exclusively via a GPI anchor, classifying it as a lipoprotein.
• Keywords associated with CD52 include direct protein sequencing, 3D-structure, and reference proteome, reflecting its well-characterized biochemical properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–61) of human CD52, which is extracellular and exposed on intact cells, supporting the antibody's suitability for live-cell flow cytometry.
• As a PE-conjugated primary antibody, no secondary staining is required; direct detection allows for simplified multicolor panel design in flow cytometry experiments.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type and instrument, and include appropriate isotype controls to assess non-specific binding.
• Validate antibody reactivity on known CD52-positive cells, such as human lymphocytes or epididymal epithelial cell lines, and include a negative control (e.g., CD52-knockout or non-human cells) to confirm specificity.
• For cell surface staining, incubate with the antibody at 4°C in the presence of Fc block to reduce potential background before flow cytometric acquisition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes comprehensive access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This PE-conjugated anti-human CD52 monoclonal antibody is ideal for researchers focusing on lymphocyte immunophenotyping or reproductive biology. We provide a full range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions to streamline your IVD development and research workflows.