PE Rabbit anti-Human CD5 mAb for FC - P06127

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5, also known as lymphocyte antigen T1/Leu-1, is a lymphoid-specific receptor expressed on all T cells and a subset of B cells (B1a cells).
• Functions as a negative regulator of TCR signaling during thymocyte development by interacting with LCK, CD3Z, PI3K, and CBL, and modulating downstream signaling cascades. Related references: PMID:1384049, PMID:1385158, PMID:23376399
• Modulates B-cell biology by activating ERK1/2 via TRPC1-mediated Ca2+ influx, promoting IL-10 production. Related references: PMID:27499044
• Interacts with B-cell ligands CD5L and CD72 to mediate T-dependent B-cell activation and maintain regulatory T/B-cell homeostasis.
• Predominantly localized to the cell membrane as a single-pass type I transmembrane protein.
• Heavily glycosylated and phosphorylated; contains extracellular SRCR domains and intramolecular disulfide bonds essential for structure.
• Plays a role in thymocyte selection, T-cell effector differentiation, and immune tolerance.

Experimental Guidance and Technical Tips

• This PE-conjugated rabbit monoclonal antibody is designed for flow cytometry. Validate the optimal dilution in your specific experimental system.
• PE provides bright fluorescence; consider co-staining with FITC or APC conjugates, using appropriate isotype controls and Fc blocking to reduce non-specific binding.
• For intracellular staining, optimize fixation/permeabilization protocols as CD5 is primarily a surface marker.
• The immunogen (aa 25-371, extracellular domain) ensures recognition of native CD5 on cell surfaces.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, supporting diagnostic manufacturers and researchers with validated antibodies and bulk ancillary reagents. The CD5 antibody is part of our expanding portfolio for immunophenotyping and immune monitoring assays. In addition to this PE conjugate, we can supply alternative formats (e.g., purified, biotin, APC) and large quantities to meet your clinical assay development requirements.