PE Rabbit anti-Human CD44V6 Monoclonal Antibody for FC - P16070

Core Product Specifications and Parameters

Biological Background: CD44V6 Function and Localization

• CD44 antigen is a cell-surface receptor that mediates cell-cell and cell-matrix interactions, primarily by binding hyaluronan, and regulates cell adhesion and migration. Related references: PMID:16541107, PMID:19703720, PMID:22726066
• It participates in diverse physiological processes including T-lymphocyte homing and activation, hematopoiesis, and inflammatory responses. Related references: PMID:7528188
• CD44 ectodomain interacts with extracellular ligands such as hyaluronan, collagen, and growth factors, while its intracellular domain assembles signaling complexes containing kinases and RhoGTPases, thereby linking extracellular cues to cytoskeletal reorganization and migration. Related references: PMID:18757307, PMID:23589287, PMID:15123640
• Alternative splicing of the CD44 gene produces multiple isoforms, including variants that contain additional peptide sequences encoded by variant exons (e.g., v6). These isoforms exhibit distinct tissue distribution and functions; the CD44V6 isoform is notably associated with epithelial cells and carcinomas.
• The protein localizes to the cell membrane and microvilli, and can be released as a soluble ectodomain by proteolysis.
• Post-translational modifications include extensive glycosylation, phosphorylation, and heparan sulfate proteoglycan modification, which regulate its function.
• Interaction with LGALS9 activates NK cells by stimulating LCK-ERK-MAPK signaling pathways. Related references: PMID:37006235
• CD44 also serves as a receptor for certain pathogens; it facilitates foot-and-mouth disease virus entry via macropinocytosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a specific region (aa 223-267) within the CD44V6 variant; consider verifying isoform specificity by comparing staining on cells expressing different CD44 splice forms.
• This antibody is validated for flow cytometry (FC). When performing FC, titrate the antibody to determine the optimal concentration for your cell type and experiment. Include proper isotype controls and Fc receptor blocking to minimize non-specific binding.
• CD44V6 expression may be upregulated on activated lymphocytes and certain carcinoma cells. Use appropriate positive (e.g., activated T cells) and negative controls to assess staining specificity.
• Note that CD44 can be proteolytically shed from the cell surface; prolonged incubation or cell activation may influence the detectable membrane-bound population.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents to diagnostic manufacturers and researchers. This PE Rabbit anti-Human CD44V6 mAb is part of our comprehensive portfolio supporting immunophenotyping and oncology research, with reliable supply for large-scale production. Partner with CamelBio to access rare target raw materials and custom solutions that accelerate your diagnostic development from concept to clinic.