PE Rabbit anti-Human CD43 Monoclonal Antibody for FC - P16150

Core Product Specifications and Parameters

Biological Background: CD43 Function and Localization

• Leukosialin (CD43, sialophorin, GPL115) is the major cell surface sialoprotein of leukocytes, encoded by the SPN gene. It is a heavily glycosylated type I transmembrane protein.
• CD43 regulates multiple T-cell functions including activation, proliferation, differentiation, trafficking and migration. It positively regulates T-cell trafficking to lymph nodes via association with ERM proteins (ezrin, radixin, moesin) (By similarity).
• CD43 biases T-cell differentiation towards a Th1 lineage by negatively regulating Th2 cell differentiation and promoting IFN-gamma expression during TCR activation of naive cells (PubMed:18036228). Related references: PMID:18036228
• CD43 prepares T-cells for cytokine sensing and effector differentiation by inducing expression of cytokine receptors IFNGR and IL4R, promoting their signaling, and mediating clustering of IFNGR with TCR (PubMed:24328034). Related references: PMID:24328034
• Acts as a major E-selectin ligand on Th17 cells, mediating their rolling on activated vasculature and recruitment during inflammation, but not on Th1 cells. Also acts as a T-cell counter-receptor for SIGLEC1 (By similarity).
• Protects cells from apoptotic signals, promoting cell survival (By similarity).
• Subcellular localization includes the plasma membrane, microvilli, uropodium, and can also localize to the nucleus in PML bodies. It is expressed on thymocytes, T-lymphocytes, neutrophils, plasma cells, and myelomas.
• Post-translational modifications: heavily O-glycosylated and sialylated; also phosphorylated and ubiquitinated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human CD43, which is extracellular; the antibody likely recognizes the extracellular domain, making it suitable for detection of native surface CD43 by flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions. Use appropriate isotype controls and Fc blocking reagents.
• CD43 is highly expressed on most leukocytes; consider including lineage markers for gating specific lymphocyte subpopulations (e.g., T cells, neutrophils) when analyzing heterogeneous samples.
• Since the antibody is PE-conjugated, avoid using other PE-conjugated antibodies in the same panel; verify compensation settings if multiplexing with other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to a comprehensive portfolio of IVD raw materials, including this PE-conjugated anti-CD43 antibody for immunology and leukocyte profiling applications. Whether you need validated antibody pairs, bulk ancillary reagents, or support sourcing rare targets like leukosialin, we provide one-stop procurement solutions backed by molecular biology and B2B supply expertise.