PE Rabbit anti-Human CD42b Monoclonal Antibody for Flow Cytometry - P07359

Core Product Specifications and Parameters

Biological Background: CD42b Function and Localization

• Platelet glycoprotein Ib alpha chain (GP1BA/CD42b) is a surface membrane protein and major subunit of the GPIb-V-IX complex.
• It plays a critical role in primary hemostasis by mediating platelet adhesion to von Willebrand factor (vWF) at sites of vascular injury.
• Contains leucine-rich repeats (LRRs) which are important for protein-protein interactions.
• Localizes to the plasma membrane of platelets.
• Undergoes post-translational modifications including phosphorylation, glycosylation, and sulfation.
• Mutations in GP1BA are associated with bleeding disorders such as Bernard-Soulier syndrome and von Willebrand disease.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is designed for direct flow cytometry detection of CD42b on human platelets or CD42b-expressing cells.
• The immunogen corresponds to the extracellular domain (amino acids 15–531), ensuring recognition of the native membrane-bound form.
• Validate in human platelet samples or relevant cell lines; consider blocking and isotype controls to minimize nonspecific binding.
• For flow cytometry, titrate the antibody to determine optimal concentration and acquire data with appropriate gating strategies.
• Protect conjugated antibody from light; avoid repeated freeze-thaw cycles to maintain performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to streamline sourcing of IVD raw materials. This anti-CD42b antibody is ideal for hemostasis and platelet disorder studies; we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whatever your target or application, we offer customized procurement support to accelerate development from concept to clinic.