PE Rabbit anti-Human CD40 mAb for FC - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40, also known as tumor necrosis factor receptor superfamily member 5 (TNFRSF5), is a cell surface receptor for CD40LG/TNFSF5. It transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. Related references: PMID:31331973
• Alternative splicing produces a secreted isoform, in addition to the canonical membrane-bound receptor.
• Localizes primarily to the cell membrane but can also be secreted. The mature protein has a molecular weight of approximately 31 kDa (calculated 30.6 kDa).
• Contains characteristic cysteine-rich repeats, disulfide bonds, and N-linked glycosylation sites, which are critical for ligand binding and receptor stability.
• Expressed on B-cells and in primary carcinomas, reflecting its dual role in adaptive immunity and tumor biology.
• CD40 signaling is essential for B-cell maturation, class switching, and germinal center formation; its dysregulation is implicated in autoimmune diseases and cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21–193); thus, the antibody is suitable for detecting cell surface CD40 by flow cytometry without permeabilization.
• Consider titrating the antibody in the relevant cell system to determine the optimal concentration that balances signal-to-noise ratio.
• Include appropriate controls such as isotype-matched PE-conjugated antibody and CD40-negative cells to confirm specificity.
• Protect the PE conjugate from prolonged exposure to light and avoid repeated freeze-thaw cycles to maintain fluorescence intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering this PE-conjugated anti-CD40 monoclonal antibody as part of a comprehensive portfolio to support immunology and oncology research. Whether you require validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, or rare target sourcing, CamelBio’s supply solutions help diagnostic developers streamline every stage from concept to clinic.