PE Rabbit anti-Human CD361 Monoclonal Antibody for FC - P34910

Core Product Specifications and Parameters

Biological Background: EVI2B / CD361 Function and Localization

• Encoded by the EVI2B gene, CD361 is a transmembrane glycoprotein also known as Ecotropic viral integration site 2B protein homolog, with alternative splicing generating multiple isoforms.
• Plays an essential role in granulocyte differentiation and the functionality of hematopoietic progenitor cells, influencing cell cycle progression and survival.
• Localizes to the membrane as a single-pass transmembrane helix protein, consistent with its involvement in extracellular signaling or adhesion.
• Post-translational modifications include N-linked glycosylation and phosphorylation, which may regulate its stability, localization, or signaling functions.
• Classified as a proto-oncogene; its dysregulation is linked to hematopoietic malignancies, although the precise oncogenic mechanisms remain under investigation.
• Broadly expressed in bone marrow, peripheral blood mononuclear cells, fibroblasts, and EBV-transformed lymphoblastoid lines; shows strong expression in granulocytic cells and weaker expression on lymphocytes.
• Contains a signal peptide for targeting to the secretory pathway and features a transmembrane domain required for membrane anchoring.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (amino acids 22–199) of CD361, making this antibody well-suited for detecting surface-expressed protein in live-cell flow cytometry assays.
• For FC, consider titrating the antibody to determine the optimal concentration for your specific cell type and experimental setup; a starting range of 0.5–5 µg per million cells is often useful for conjugated antibodies.
• Validate the antibody in the relevant sample system (e.g., human PBMCs, granulocytic cell lines) and include appropriate controls, such as unstained cells and isotype-matched PE-conjugated antibodies, to ensure specificity.
• Because CD361 expression is strongest in granulocytes, pre-gating on granulocytic markers or using density centrifugation to enrich granulocytes may enhance signal resolution.
• Sample preparation conditions (e.g., fixation, permeabilization) should be optimized for surface staining if viability is affected, though live-cell staining is recommended for this directed-against-extracellular-domain antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with one-stop access to premium IVD raw materials and technical support. This PE Rabbit anti-Human CD361 monoclonal antibody exemplifies our commitment to delivering high-quality reagents for hematopoietic cell analysis—whether you need validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, or rare-target raw material sourcing, our platform bridges the gap from concept to clinic efficiently.