PE Rabbit anti-Human CD352/SLAMF6 Monoclonal Antibody for FC - Q96DU3

Core Product Specifications and Parameters

Biological Background: SLAMF6 Function and Localization

• SLAMF6 is a self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family, modulating activation and differentiation of various immune cells. Related references: PMID:11489943 PMID:16920955
• In natural killer (NK) cells, it triggers cytolytic activity when expressed at high densities of natural cytotoxicity receptors. Related references: PMID:11489943 PMID:16920955
• Positive signaling in NK cells involves phosphorylation of VAV1 and depends on the adapter SH2D1B/EAT-2. Related references: PMID:16920955
• SLAMF6 promotes T-cell differentiation into a Th17 phenotype, enhancing IL-17 secretion; this costimulatory activity requires SH2D1A/SAP. Related references: PMID:16920955 PMID:22184727
• It facilitates recruitment of RORC to the IL-17 promoter. Related references: PMID:22989874
• SLAMF6 also may act as a negative regulator of humoral immunity, inhibiting T-cell:B-cell adhesion and germinal center formation (By similarity).
• The protein is expressed on the surface of resting and activated NK cells, T- and B-lymphocytes, with increased surface expression on T-cells from systemic lupus erythematosus patients. Related references: PMID:11489943 PMID:22184727
• Subcellular localization is the cell membrane; it is a single-pass type I membrane protein with an immunoglobulin domain.

Experimental Guidance and Technical Tips

• As a PE-conjugated antibody, protect from light and store according to manufacturer recommendations to prevent fluorophore degradation.
• For flow cytometry, titrate the antibody to determine the optimal working concentration for your cell population and staining conditions.
• The immunogen corresponds to the extracellular domain (aa 24-215), ensuring recognition of the native cell surface receptor.
• The predicted molecular weight is 37 kDa, but due to glycosylation and alternative splicing, bands between 24 and 37 kDa may be observed in non-FC applications like Western blot; validate accordingly.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline your IVD development by providing a comprehensive range of high-quality raw materials, from optimized antibodies to bulk reagents. This anti-CD352/SLAMF6 antibody supports immune cell research and can be integrated into assay development with our full suite of immunological reagents. Contact our team for assistance with custom sourcing and technical consultation.