PE Rabbit anti-Human CD34 Monoclonal Antibody for Flow Cytometry - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• Hematopoietic progenitor cell antigen CD34 is a single-pass type I transmembrane glycoprotein encoded by the CD34 gene in humans, with multiple isoforms produced by alternative splicing.
• It serves as a possible adhesion molecule with a critical role in early hematopoiesis, mediating attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells.
• CD34 may act as a scaffold for lineage-specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components, and presents carbohydrate ligands to selectins.
• The protein is selectively expressed on the surface of hematopoietic progenitor cells and on the small vessel endothelium of a variety of tissues.
• Subcellularly, CD34 is localized to the membrane, consistent with its function as a cell surface receptor involved in cell adhesion and signaling.
• Post-translational modifications include phosphorylation and extensive glycosylation, contributing to the observed protein weight of approximately 35–41 kDa versus the predicted molecular weight of ~41 kDa from sequence analysis.
• Keywords associated with CD34 include alternative splicing, cell adhesion, glycoprotein, membrane, phosphoprotein, and transmembrane helix, reflecting its structural and functional characteristics.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 170–290 of human CD34, a region within the extracellular domain that is likely accessible for binding and may confer specificity for native conformations.
• This PE-conjugated rabbit monoclonal antibody is designed for flow cytometry. For optimal results, titrate the antibody in the relevant sample system and include appropriate isotype controls to assess background staining.
• Consider employing gentle cell handling and staining protocols to preserve surface CD34 expression, as it may be sensitive to enzymatic or mechanical disruption.
• If performing multicolor flow cytometry, verify compatibility with other fluorophores to avoid spectral overlap, particularly with PE tandem dyes.
• Given the reported protein weights of 35 kDa and 41 kDa, likely reflecting different glycosylation states, validate the antibody’s performance in your specific cell type and experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic manufacturers and research labs. This PE Rabbit anti-Human CD34 monoclonal antibody supports hematopoietic stem cell research and flow cytometry applications, complementing our sourcing capabilities for rare targets and custom antibody development. Partner with CamelBio for efficient, end-to-end supply solutions that accelerate your projects from concept to clinic.