PE Rabbit anti-Human CD336/NK-p44 mAb for FC - O95944

Core Product Specifications and Parameters

Biological Background: CD336/NK-p44 Function and Localization

• CD336/NK-p44, also known as Natural cytotoxicity triggering receptor 2 (NCR2), lymphocyte antigen 95 homolog, or NKp44, is a member of the immunoglobulin superfamily that acts as an activating receptor on natural killer (NK) cells.
• It plays a role in enhancing the cytotoxicity of activated NK cells, contributing to tumor cell lysis.
• The receptor is selectively expressed on the surface of activated NK cells and in vitro cultured TCRγ/δ lymphoid cells.
• Subcellularly, NCR2 is a single-pass type I membrane protein localized to the cell membrane.
• It contains an extracellular Ig-like V-type domain, a transmembrane helix, and a short cytoplasmic tail; the gene undergoes alternative splicing.
• Post-translational modifications include glycosylation and disulfide bond formation, critical for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 22-190 of the human CD336/NK-p44 extracellular domain, suggesting recognition of surface-exposed epitopes suitable for flow cytometry.
• For FC, titrate the antibody and include appropriate isotype controls; activation of NK cells (e.g., with IL-2 or IL-15) may be necessary to induce CD336 expression for detection.
• As a PE-conjugated antibody, ensure proper compensation and panel design when combining with other fluorophores in multicolor flow cytometry experiments.
• The antibody is specific for human CD336; cross-reactivity with other species is not expected, so validate in the relevant human sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development by providing this PE-conjugated anti-human CD336 monoclonal antibody as part of a comprehensive raw-material portfolio, including validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. Whether you are building NK cell-based assays or investigating tumor immune evasion, our one-stop sourcing model streamlines procurement from concept to clinic, with technical consulting available to accelerate your diagnostic development.