PE Rabbit anti-Human CD335/NKp46 Monoclonal Antibody for FC - O76036

Core Product Specifications and Parameters

Biological Background: CD335/NKp46 Function and Localization

• CD335, also known as Natural cytotoxicity triggering receptor 1 (NCR1), lymphocyte antigen 94 homolog, NK-p46, or NKp46, is a transmembrane receptor belonging to the immunoglobulin superfamily.
• It functions as a cytotoxicity-activating receptor on natural killer (NK) cells, contributing to the enhanced ability of activated NK cells to mediate tumor cell lysis.
• The protein is selectively expressed on the surface of both resting and activated NK cells, highlighting its role as a key marker for NK cell identification.
• CD335 is localized to the cell membrane and possesses an extracellular region with immunoglobulin-like domains and a cytoplasmic tail involved in signal transduction.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which are critical for proper folding and function.
• Multiple protein isoforms are produced by alternative splicing, with molecular weights ranging from approximately 21 kDa to 34 kDa as reported in various databases.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the extracellular domain (amino acids 22-254), making this antibody well-suited for detecting native surface CD335 on live cells by flow cytometry.
• Optimal working concentration should be determined by titration in the specific experimental system, considering cell type, target density, and instrument settings.
• Since NKp46 expression is restricted to NK cells, ensure proper gating and consider co-staining with other NK lineage markers (e.g., CD56, CD16) to confirm specificity.
• For intracellular staining or detection in fixed permeabilized cells, verify compatibility with the fixation/permeabilization protocol to prevent epitope masking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, research laboratories, and clinical institutions with streamlined access to high-quality IVD raw materials and technical support. As a dedicated partner in the immunology and oncology research arena, we offer an extensive portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for molecules like CD335/NKp46. Trust CamelBio to accelerate your NK cell–based assay development from concept to commercialization with reliable, integrated supply solutions.