PE Rabbit anti-Human CD33 Monoclonal Antibody for FC - P20138

Core Product Specifications and Parameters

Biological Background: CD33 Function and Localization

• CD33 is a sialic-acid-binding immunoglobulin-like lectin (Siglec-3) that mediates cell-cell interactions and maintains immune cells in a resting state. Related references: PMID:10611343, PMID:11320212, PMID:15597323

• It preferentially recognizes and binds alpha-2,3- and alpha-2,6-linked sialic acid-bearing glycans. Related references: PMID:7718872

• Ligand engagement triggers phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) by Src-like kinases (e.g., LCK), creating docking sites for protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. Related references: PMID:10887109, PMID:28325905, PMID:10206955, PMID:10556798

• These phosphatases regulate downstream pathways through dephosphorylation of signaling molecules; one repressive effect on monocytes requires phosphoinositide 3-kinase/PI3K. Related references: PMID:10206955, PMID:10887109, PMID:15597323

• CD33 localizes to the cell membrane and has been reported in peroxisomes.

• It is predominantly expressed on monocytic/myeloid lineage cells and, in the brain, on microglial cells.

• Annotated keywords include glycoprotein, disulfide bond, alternative splicing, and availability of a 3D-structure.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is designed for flow cytometry; consider titrating to determine the optimal working concentration for your cell system.
• CD33 is expressed on human monocytic/myeloid lineage cells; validate staining using appropriate positive (e.g., monocytes) and negative cell populations.
• To minimize non-specific binding, include an Fc receptor blocking step prior to staining.
• The immunogen covers the extracellular domain (aa 18-259), which may influence recognition of native versus denatured CD33 in other applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including this anti-CD33 monoclonal antibody, to support your immunology and oncology research. From validated antibody pairs to bulk ancillary reagents, we provide one-stop procurement solutions for diagnostic manufacturers and laboratories. Contact us to explore customized sourcing for rare targets and large-scale production needs.