PE Rabbit anti-Human CD32 mAb - P12318

Core Product Specifications and Parameters

Biological Background: CD32 (FCGR2A) Function and Localization

• CD32 (FCGR2A) is a low affinity immunoglobulin gamma Fc region receptor II-a, also designated CDw32 or FcγRIIa, belonging to the immunoglobulin superfamily.
• Encoded by the FCGR2A gene on chromosome 1, multiple isoforms are produced through alternative splicing, including FCGR2A1.
• It binds to the Fc region of IgG with low affinity, mediating immune responses against pathogens and soluble antigens.
• Upon IgG binding, CD32 triggers phagocytosis of opsonized antigens, crucial for pathogen clearance and antigen presentation.
• Constitutively expressed on the surface of monocytes, neutrophils, and eosinophils, as well as on platelets.
• Subcellularly, CD32 localizes to the cell membrane as a single-pass type I transmembrane protein.
• Key post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation, which regulate receptor function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 36–218) of human CD32, suggesting the antibody is suitable for detecting native cell surface receptor by flow cytometry.
• For flow cytometry (FC) applications, titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Given expression on monocytes, neutrophils, and eosinophils, incorporate appropriate gating strategies and include isotype controls to ensure specific detection.
• Consider using this antibody in combination with other lineage markers to characterize immune cell subsets in human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw material sourcing, supporting diagnostic manufacturers and research labs from concept to commercialization. This PE-conjugated anti-CD32 monoclonal antibody is optimized for flow cytometry, enabling immune phenotyping and functional studies in immunology and infectious disease research. Beyond this product, we offer validated antibody pairs, bulk monoclonals and polyclonals, ancillary reagents, and custom sourcing for rare targets to streamline your assay development pipeline.