PE Rabbit anti-Human CD300 a/c mAb for FC - Q9UGN4 / Q08708

Core Product Specifications and Parameters

Biological Background: CD300a/c Function and Localization

• CD300a, also designated CMRF35-like molecule 8, CMRF35-H9, IRp60, or IgSF12, is an inhibitory receptor of the CD300 family of immunoglobulin-like proteins.
• It functions as an inhibitory receptor that down-regulates cytolytic activity in natural killer (NK) cells and mast cell degranulation. Related references: PMID:10746781 PMID:16339535 PMID:9701027
• CD300a negatively regulates Toll-like receptor (TLR) signaling mediated by MYD88, but not TRIF, through activation of the phosphatase PTPN6. Related references: PMID:22043923
• The protein is broadly expressed on immune cells including NK cells, T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes, and monocytes.
• Subcellularly, CD300a localizes to the cell membrane as a single-pass type I transmembrane protein containing an extracellular immunoglobulin-like domain.
• Key structural features include disulfide bonds, N-linked glycosylation, and phosphorylation sites, consistent with its role in cell adhesion and immune regulation.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 18–178) of CD300a, sharing homology with CD300c; this PE-conjugated antibody is designed for flow cytometry (FC) to detect cell surface CD300a/c on live human immune cells.
• For FC applications, perform antibody titration to determine the optimal working concentration for your specific cell type and experimental system.
• Use appropriate isotype controls and viability dyes to ensure specific staining and exclude dead cells.
• Because CD300a is expressed on multiple leukocyte subsets, consider co-staining with lineage markers (e.g., CD3, CD19, CD56) for population-specific analysis.
• Validation in the relevant sample system (e.g., PBMCs, purified NK cells) is recommended to confirm reactivity and performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials and customized sourcing solutions for diagnostic manufacturers, laboratories, and research institutes. For CD300a/c research and assay development, we provide validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents to support immunophenotyping and functional studies. Contact us to explore rare-target sourcing and bulk procurement options that accelerate your project from concept to clinic.