PE Rabbit anti-Human CD30 Monoclonal Antibody for FC - P28908

Core Product Specifications and Parameters

Biological Background: CD30 Function and Localization

• CD30, also designated TNFRSF8, is a member of the tumor necrosis factor receptor superfamily and is known as Ki-1 antigen and lymphocyte activation antigen.
• It acts as a receptor for TNFSF8/CD30L (PubMed:8391931) and may regulate cellular growth and transformation of activated lymphoblasts. Related references: PMID:8391931
• CD30 activates NF-kappa-B signaling, thereby modulating gene expression (PubMed:8999898). Related references: PMID:8999898
• The gene encodes multiple protein isoforms via alternative initiation and alternative splicing.
• CD30 localizes to the cell membrane and cytoplasm, consistent with its function as a transmembrane receptor.
• At the protein level, CD30 has been detected in alveolar macrophages.
• Post-translational modifications include disulfide bond formation, glycosylation, and phosphorylation; the protein contains a signal peptide, extracellular repeat domains, a transmembrane helix, and a cytoplasmic tail.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 19-379) corresponds to the extracellular region of CD30, making this PE-conjugated antibody suitable for surface staining of live cells in flow cytometry.
• Consider testing the antibody on activated lymphocytes or alveolar macrophages, where CD30 expression has been reported.
• For intracellular detection, a permeabilization step is recommended due to the cytoplasmic localization of CD30.
• Optimal concentration and staining conditions should be determined by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, connecting diagnostic developers and researchers with high-quality reagents such as this anti-CD30 monoclonal antibody. Our product range includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, all tailored to support immunology and oncology applications. With expertise in rare target sourcing, we streamline your path from discovery to diagnostic production.