PE Rabbit anti-Human CD282/TLR2 Monoclonal Antibody for Flow Cytometry - O60603

Core Product Specifications and Parameters

Biological Background: TLR2 Function and Localization

• TLR2 (Toll-like receptor 2, also known as TIL4 or CD282) is a key pattern recognition receptor that mediates innate immune responses to bacterial lipoproteins and other microbial cell wall components. It cooperates with LY96 and uses MYD88 and TRAF6 adaptors to trigger NF-kappa-B activation and pro-inflammatory cytokine secretion. Related references: PMID:10426995, PMID:10426996
• TLR2 forms functional heterodimers with TLR1 or TLR6 to discriminate between triacylated and diacylated lipopeptides, respectively, and recognizes mycoplasmal MALP-2, bacterial soluble factors, and Borrelia OspA lipoprotein. Related references: PMID:11441107
• The receptor localizes to the cell membrane, cytoplasmic vesicles, and phagosome membranes, and is enriched in lipid raft microdomains for signaling complex assembly.
• TLR2 is highly expressed in peripheral blood leukocytes, particularly monocytes, and in bone marrow, lymph node, and spleen, with lower levels in lung and fetal liver.
• Post-translational modifications include N-glycosylation, disulfide bond formation, and ubiquitination (Ubl conjugation).
• It recognizes multiple Mycobacterium tuberculosis components, such as lipoproteins LprA, LprG, LpqH, PstS1, and HSP70 (dnaK), and triggers MAP kinase (p38, ERK1/2) pathways. Related references: PMID:16622205, PMID:19362712, PMID:15809303
• Ligand-induced activation clusters containing TLR2, TLR1/6, CD14, and CD36 are formed at the cell surface and internalized to the Golgi, a process important for fine-tuning innate immune signaling. Related references: PMID:16880211

Experimental Guidance and Technical Tips

• As a PE-conjugated antibody, this reagent is ready for direct immunofluorescence staining in flow cytometry (FC) without a secondary detection step. Consider using an appropriate PE-labeled isotype control and performing titration to optimize the signal-to-noise ratio.
• The immunogen corresponds to the extracellular domain (aa 290–390) of human TLR2, making the antibody suitable for surface staining of live cells. Validate staining efficiency in relevant human leukocyte populations such as peripheral blood monocytes or PBMCs.
• Protect the PE conjugate from prolonged light exposure during handling and staining to maintain fluorophore performance.
• TLR2 expression can be upregulated upon immune activation; the antibody may be used to monitor innate immune status in experimental models or clinical samples.
• Cross-reactivity has been confirmed for human; performance in other species has not been tested. Validate in the appropriate sample system if non-human models are used.

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CamelBio is a one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers and research laboratories with direct access to high-quality reagents like this anti-TLR2 monoclonal antibody. We support innate immunity and infectious disease research with validated antibody pairs, recombinant proteins, and ancillary reagents. Contact us for bulk orders and tailored sourcing solutions that cover every stage from concept to clinic.