PE Rabbit anti-Human CD275/B7-H2/ICOSL Monoclonal Antibody for FC - O75144

Core Product Specifications and Parameters

Biological Background: ICOS ligand Function and Localization

• Functions as a ligand for the T-cell-specific surface receptor ICOS, delivering a costimulatory signal essential for T-cell proliferation and cytokine secretion.
  Related references:
  PMID:11007762
  PMID:11023515
  PMID:30498080

• Induces B-cell proliferation and differentiation into plasma cells, contributing to humoral immunity.

• May play an important role in local tissue responses to inflammatory conditions and in modulating secondary immune responses by co-stimulating memory T-cell function (By similarity).

• In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants such as CXCL8/IL8 and fMLP.
  Related references:
  PMID:30498080

• Localizes to the cell membrane as a type I transmembrane protein.

• Expressed on peripheral blood B-cells and monocytes, monocyte-derived dendritic cells, and widely in lymphoid and non-lymphoid tissues including lymph nodes, spleen, leukocytes, and on activated monocytes/dendritic cells.

• Predicted structural features include an immunoglobulin domain, disulfide bonds, N-linked glycosylation, and a signal peptide, with multiple isoforms produced by alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 19-256) encompasses the extracellular domain, consistent with detection of cell-surface ICOS ligand in flow cytometry (FC). Consider optimizing the antibody concentration by titration on a relevant cell line or primary cells known to express ICOSLG.

• For FC, include appropriate PE-conjugated isotype controls to assess background, and use viable cell gating to exclude dead cells which may non-specifically bind the conjugate.

• ICOSLG expression is inducible on activated monocytes and dendritic cells; thus, stimulation protocols may be required to detect the target in resting cell populations.

• Predicted bands on western blot may appear at approximately 20, 33, and 34 kDa due to glycosylation and splice variants; however, this product is validated only for FC; verify suitability in other applications by independent testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing of IVD raw materials, supporting your initiatives in immune checkpoint research and adaptive immunity. Beyond this CD275/B7-H2/ICOSL monoclonal antibody, we provide validated antibody pairs, optimized antibodies, bulk ancillary reagents, and custom sourcing of rare targets — streamlining your path from concept to clinic.