PE Rabbit anti-Human CD270/HVEM Monoclonal Antibody for FC - Q92956

Core Product Specifications and Parameters

Biological Background: Tumor necrosis factor receptor superfamily member 14 Function and Localization

• Tumor necrosis factor receptor superfamily member 14 (TNFRSF14), also known as HVEM, CD270, ATAR, and TR2, is encoded by the TNFRSF14 gene. Alternative splicing and post‑translational modifications yield isoforms with observed molecular weights of approximately 21 kDa and 30 kDa.
• Serves as a receptor for multiple ligands: the TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA, and the immunoglobulin superfamily members BTLA and CD160. Signals via the TRAF2‑TRAF3 E3 ligase pathway to promote immune cell survival and differentiation. Related references: PMID:19915044, PMID:9153189, PMID:9162022
• Upon LIGHT binding, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions. Related references: PMID:10754304
• Interaction with CD160 on NK cells enhances IFNG production and anti‑tumor immune responses. On activated CD4+ T cells, binding to CD160 down‑regulates CD28 costimulatory signaling, restricting memory and alloantigen‑specific immune responses. Related references: PMID:23761635, PMID:18193050
• Participates in bidirectional cell‑cell contact signaling between antigen presenting cells and lymphocytes. Can engage BTLA in cis (on the same cell) or in trans (on other cells), differentially regulating T cell resting states and survival signals. Related references: PMID:19915044
• Localizes to the cell membrane. Widely expressed, with highest levels in lung, spleen, and thymus; also found on a subpopulation of B cells, monocytes, and naive T cells. Related references: PMID:18193050, PMID:19915044
• Acts as a host cell receptor for herpes simplex virus 1 (HHV‑1) and herpes simplex virus 2 (HHV‑2), mediating viral entry.
• Post‑translational modifications include disulfide bond formation, glycosylation, and phosphorylation; the protein contains a signal peptide and a transmembrane helix.

Experimental Guidance and Technical Tips

• This PE‑conjugated rabbit monoclonal antibody is designed for direct flow cytometry detection of human CD270/HVEM on the cell surface.
• The immunogen corresponds to the extracellular domain (amino acids 39–162), confirming suitability for surface staining protocols.
• Use appropriate isotype controls and Fc receptor blocking to minimize nonspecific binding.
• Validate performance on relevant human cell types such as PBMCs, lymphoid tissues, or CD270‑expressing cell lines.
• Consider the presence of soluble receptor shedding in culture systems; include appropriate controls or fresh sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated monoclonal antibodies like this PE‑conjugated anti‑CD270/HVEM reagent. Our portfolio supports immuno‑oncology and virology research, covering immune checkpoint targets and host‑pathogen interactions. We streamline procurement with offerings such as validated antibody pairs, bulk ancillary reagents, and custom rare‑target sourcing—helping you advance from concept to clinic efficiently.