PE Rabbit anti-Human CD262/DR5/TRAILR2 Monoclonal Antibody for Flow Cytometry - O14763

Core Product Specifications and Parameters

Biological Background: CD262/DR5/TRAILR2 Function and Localization

• Receptor for TNFSF10/TRAIL, forming a death-inducing signaling complex (DISC) with FADD and caspase-8 to trigger apoptosis. Related references: PMID:10549288
• Promotes NF-kappa-B activation, linking death receptor signaling to inflammatory and survival pathways.
• Essential for ER stress-induced apoptosis, mediating cellular responses to misfolded protein accumulation.
• Subcellular localization: Membrane; single-pass type I transmembrane protein with an extracellular ligand-binding domain.
• Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines (HeLa, K-562, HL-60, SW480, A-549, G-361); not detectable in brain.
• Post-translational modifications include glycosylation and disulfide bond formation, critical for receptor folding and function.
• Alternative splicing generates multiple isoforms, potentially modulating TRAIL sensitivity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 56–182), ensuring recognition of the native membrane-bound receptor.
• PE conjugation enables direct detection in flow cytometry (FC) without secondary labeling steps. Consider titrating the antibody (e.g., 0.5–5 µL per test) to optimize signal-to-noise ratio.
• For best results, validate the antibody in your specific sample system, using known TRAIL-R2–positive human cell lines (e.g., HeLa, K-562) as positive controls and appropriate isotype controls.
• Protect the conjugate from light and avoid freeze-thaw cycles to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research manufacturers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-human CD262/DR5/TRAILR2 antibody is part of our apoptosis and oncology offerings, backed by reliable access to rare-target raw materials and technical guidance—helping you move from concept to clinic efficiently.