PE Rabbit anti-Human CD261/TRAIL-R1 Monoclonal Antibody for FC - O00220

Core Product Specifications and Parameters

Biological Background: CD261/TRAIL-R1 Function and Localization

• CD261/TRAIL-R1 (TNFRSF10A), also known as Death Receptor 4 (DR4), is a cell surface receptor for the cytotoxic ligand TNFSF10/TRAIL. Related references: PMID:26457518, PMID:38532423
• Upon TRAIL binding, the receptor trimerizes and recruits the adapter molecule FADD, which in turn recruits and activates caspase-8, forming the death-inducing signaling complex (DISC) to initiate apoptosis. Related references: PMID:19090789
• TRAIL-R1 also promotes the activation of NF-kappa-B signaling, linking death receptor engagement to inflammatory and survival pathways. Related references: PMID:9430227
• The receptor is widely expressed, with high levels observed in spleen, peripheral blood leukocytes, small intestine, thymus, and certain tumor cells such as K-562 and MCF-7, as well as activated T-cells.
• Subcellularly, TRAIL-R1 localizes to the cell membrane and membrane rafts, but can also be found in the cytosol, reflecting its dynamic trafficking upon stimulation.
• Key structural features include an extracellular cysteine-rich domain, a single transmembrane helix, disulfide bonds, and glycosylation sites; post-translational modifications such as methylation and phosphorylation may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 124–232), suggesting the antibody likely recognizes an extracellular epitope, making it suitable for flow cytometry without permeabilization.
• For FC, consider titrating the antibody to determine the optimal concentration for your cell type and experimental conditions.
• Use appropriate isotype controls and viability dyes to ensure specific and accurate detection of CD261/TRAIL-R1 on live cells.
• Validate the antibody’s reactivity in your experimental model system and check for any nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-human CD261/TRAIL-R1 antibody supports apoptosis and death receptor research; we also offer sourcing for rare targets and custom development to bridge your projects from concept to clinic.