PE Rabbit anti-Human CD244/2B4 Monoclonal Antibody for FC - Q9BZW8

Core Product Specifications and Parameters

Biological Background: CD244/2B4 Function and Localization

• CD244, also known as 2B4, is a member of the signaling lymphocytic activation molecule (SLAM) family and functions as a heterophilic receptor for CD48.
• Acts as an activating natural killer (NK) cell receptor. Related references: PMID:10359122, PMID:11714776, PMID:8376943
• Its activating function involves association with SH2D1A/SAP and FYN, leading to downstream signaling primarily through VAV1, with roles for INPP5D/SHIP1 and CBL. Related references: PMID:15713798, PMID:10934222
• Stimulates NK cell cytotoxicity, IFN-gamma production, and granule exocytosis. Related references: PMID:11714776, PMID:8376943
• Functions as a costimulator in NK activation by enhancing signals from other NK receptors such as NCR3 and NCR1. Related references: PMID:10741393
• At early stages of NK cell differentiation, may act as an inhibitory receptor, contributing to self-tolerance. Related references: PMID:11917118
• Localized to the cell membrane, specifically enriched in membrane rafts.
• CD244 is a glycoprotein with multiple isoforms arising from alternative splicing; it contains immunoglobulin-like domains, a transmembrane region, and participates in both innate and adaptive immunity.
• Expressed broadly in immune cells, including all NK cells, monocytes, basophils, a subset of CD8(+) T-cells, and TCR-gamma/delta+ T-cells; with high expression in spleen and peripheral blood.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (aa 22-221), which includes the Ig-like domains responsible for CD48 binding; this design is likely to support recognition of native CD244 conformation on live cells, making it suitable for flow cytometry.
• As a PE-conjugated antibody, it enables direct detection without a secondary reagent; consider titrating the antibody to establish the optimal staining index for your specific sample type.
• For flow cytometry validation, consider using human peripheral blood mononuclear cells (PBMCs) or NK cell lines (e.g., NK-92) as positive controls, and monitor CD244 expression under resting and activated conditions.
• Since the antibody is monoclonal, it may recognize a single epitope; ensure that the epitope is accessible and not masked by CD48 binding or other interactions in your assay.

CamelBio: Your One-Stop Sourcing Bridge

This PE-conjugated anti-CD244 antibody supports NK cell phenotyping and functional analysis in flow cytometry workflows. CamelBio connects diagnostic manufacturers and researchers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline IVD development. We also assist in sourcing rare-target raw materials, helping accelerate your project from concept to clinic.