PE Rabbit anti-Human CD24 Monoclonal Antibody for Flow Cytometry - P25063

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24, also known as Small Cell Lung Carcinoma Cluster 4 Antigen, is a GPI-anchored glycoprotein encoded by the CD24 gene. Alternative splicing generates multiple isoforms.
• It plays a pivotal role in cell differentiation across various cell types, with signaling triggered by lectin-like ligand binding to CD24 carbohydrates, leading to release of second messengers derived from its GPI-anchor.
• In B-cells, CD24 modulates activation responses, promotes antigen-dependent proliferation, and prevents terminal differentiation into antibody-forming cells. Related references: PMID:11313396
• CD24 associates with SIGLEC10 to selectively suppress immune responses to danger-associated molecular patterns (DAMPs) like HMGB1, HSP70, and HSP90, indicating a role in controlling autoimmunity.
• Subcellularly, CD24 is localized at the cell membrane, anchored via a GPI moiety.
• Tissue expression is predominantly on B-cells, also found on some T-cells, erythroleukemia cell lines, and small cell lung carcinoma lines, making it a marker for B-cell developmental stages and certain cancers.
• Post-translational modifications include glycosylation and GPI-anchor attachment, contributing to its variable molecular weight (~8-13 kDa depending on glycosylation and isoform).

Experimental Guidance and Technical Tips

• As a PE-conjugated antibody, store at 2-8°C protected from light and avoid freeze-thaw cycles to maintain fluorophore brightness.
• For flow cytometry, titrate the antibody to determine optimal working concentration in your experimental system; a starting dilution of 1:50-1:200 is common for PE conjugates, but optimization is essential.
• Include appropriate isotype controls and viability dyes to exclude dead cells and non-specific binding.
• Validate the antibody in a relevant human B-cell line (e.g., Raji, Daudi) or PBMCs to confirm expected surface staining pattern; a blocking step with normal serum from the host species may reduce background.
• Given the immunogen spanning the N-terminal extracellular domain (amino acids 1-80), the antibody is expected to recognize the exposed epitope on live cells; consider fixation/permeabilization effects if used in intracellular flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including PE-conjugated antibodies like this CD24 monoclonal antibody. For your immune cell analysis or oncology biomarker projects, we supply validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline your development pipeline. Contact us for rare target sourcing and technical support from concept to clinic.