PE Rabbit anti-Human CD235a/Glycophorin A Monoclonal Antibody for FC - P02724

Core Product Specifications and Parameters

Biological Background: Glycophorin-A Function and Localization

• Glycophorin-A (GYPA) is the major intrinsic membrane sialoglycoprotein of human erythrocytes, encoded by the GYPA gene (also known as GPA, MNS). Alternative splicing yields multiple isoforms, and the protein is heavily glycosylated, carrying the MN blood group determinants on its extracellular N-terminal region.
• Glycophorin A is a component of the ankyrin-1 complex, which is critical for erythrocyte membrane stability and shape (PubMed:35835865). Related references: PMID:35835865
• It is required for high-activity function of the anion exchanger SLC4A1 (band 3) and promotes its translocation to the plasma membrane.
• The extracellular domain serves as a receptor for Hepatitis A virus (HAV) and for P. falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of O-linked glycans.
• Subcellular localization is exclusively at the cell membrane, with a single-pass transmembrane topology and a short cytoplasmic tail.
• Post-translational modifications include extensive O-linked glycosylation and phosphorylation; the mature protein exhibits an apparent molecular weight of 13–16 kDa despite a theoretical mass of ~16.4 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 20-91) targets the extracellular domain, making this PE-conjugated antibody suitable for detection of native cell surface CD235a by flow cytometry without the need for permeabilization.
• PE direct conjugation eliminates the need for a secondary antibody, reducing background and simplifying staining protocols for multi-color FC panels.
• Consider titrating the antibody on erythrocyte-rich samples (e.g., whole blood, RBC lysates) or CD235a-expressing cell lines to determine the optimal working dilution.
• Use appropriate isotype controls and compensation controls to validate specificity and minimize spectral overlap in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-CD235a clone, bulk ancillary reagents, and rare target sourcing. Our expertise spans from concept to clinic, helping you accelerate erythrocyte biology, blood group antigen, or infectious disease research into reliable diagnostic solutions.