PE Rabbit anti-Human CD23 Monoclonal Antibody for FC - P06734

Core Product Specifications and Parameters

Biological Background: CD23 (Low affinity immunoglobulin epsilon Fc receptor) Function and Localization

• Identified as the low affinity immunoglobulin epsilon Fc receptor (FcεRII), also known as CD23, BLAST-2, and C-type lectin domain family 4 member J (CLEC4J).
• Functions as a receptor for IgE and for CR2/CD21, playing critical roles in regulating IgE production and B cell differentiation.
• On B cells, CD23 mediates IgE-dependent antigen uptake and presentation to T cells, facilitating adaptive immune responses. Related references: PMID:2167225
• On macrophages, upon IgE binding and antigen cross-linking, CD23 triggers intracellular killing of parasites via activation of the L-arginine-nitric oxide pathway. Related references: PMID:7544003
• Encoded by the FCER2 gene, CD23 belongs to the C-type lectin superfamily, containing a carbohydrate recognition domain.
• Subcellularly localized to the cell membrane as a type II transmembrane protein; also found as a secreted form (sCD23) generated by proteolytic cleavage.
• Post-translational modifications include glycosylation, disulfide bond formation, and palmitoylation; the receptor binds calcium and IgE.
• Tissue specificity: detected in urine at the protein level; broadly expressed on B cells, monocytes, and other immune cells.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (aa 48–321) of human CD23, which includes the lectin and IgE-binding regions; this design likely targets functional epitopes relevant for receptor-ligand interaction studies.
• For flow cytometry applications, validate the antibody’s performance in your specific experimental system, including appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• As a PE conjugate, ensure compensation settings are optimized when combining with other fluorophores; consider using a viability dye to exclude dead cells.
• While validated for FC, other applications (e.g., IHC, Western blot) have not been tested and would require thorough optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For IgE receptor and allergy research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether your application demands consistent lot-to-lot performance for clinical assays or specialized reagents for B cell biology, our streamlined procurement supports your projects from discovery to commercialization.