PE Rabbit anti-Human CD226/DNAM-1 Monoclonal Antibody for FC - Q15762

Core Product Specifications and Parameters

Biological Background: CD226 Function and Localization

• CD226, also known as DNAM-1 (DNAX accessory molecule 1), is a cell surface receptor crucial for the immune system, mediating intercellular adhesion and signaling in cytotoxic T-cells (CTLs) and natural killer (NK) cells. Related references: PMID:8673704, PMID:9712030
• As a costimulatory receptor, CD226 enhances the cytotoxicity of NK cells and CTLs upon binding to its ligands, PVR/CD155 or Nectin2/CD112, on virus-infected or tumor cells, promoting target cell killing. Related references: PMID:26755705, PMID:31253644, PMID:30591568
• Mechanistically, ligand engagement leads to phosphorylation by Src family kinases (e.g., LYN, FYN), recruitment of the adaptor protein GRB2, and activation of downstream effectors including VAV1, PI3K, PLCG1, ERK, and AKT, as well as calcium fluxes.
• CD226 is expressed on the cell surface membrane of peripheral blood T-lymphocytes and plays a key role in T-cell and NK-cell mediated immune surveillance.
• The protein belongs to the immunoglobulin superfamily and contains two Ig-like domains, disulfide bonds, and multiple glycosylation sites, characteristic of a transmembrane receptor.
• CD226 is also known by aliases PTA1, TLiSA1, and DNAM-1, and is widely studied for its role in tumor immunology and immune checkpoint pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of human CD226 (amino acids 19-247), suggesting the antibody likely recognizes an epitope accessible on live cells, making it potentially suitable for flow cytometric analysis of surface CD226 expression.
• When using this PE-conjugated antibody for flow cytometry, it is recommended to titrate the antibody to determine the optimal staining concentration for your specific cell type and conditions.
• Include appropriate isotype controls and Fc receptor blocking to ensure specific staining.
• CD226 is expressed on T cells and NK cells; therefore, co-staining with lineage markers (e.g., CD3, CD56) can facilitate multiparametric analysis.
• For intracellular staining, validation may be required as the epitope might be denatured after fixation/permeabilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides end-to-end sourcing solutions for IVD raw materials, supporting diagnostic manufacturers and research labs with a comprehensive portfolio of antibodies and ancillary reagents. Our offering includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk reagents for immunoassay development. For targets such as CD226/DNAM-1—critical in immune checkpoint research and cancer immunotherapy—CamelBio streamlines procurement from concept to clinic, ensuring access to reliable raw materials.