PE Rabbit anti-Human CD220 Monoclonal Antibody for Flow Cytometry - P06213

Core Product Specifications and Parameters

Biological Background: Insulin Receptor Function and Localization

• The insulin receptor (INSR, CD220) is a receptor tyrosine kinase that mediates insulin's pleiotropic actions, including metabolic and mitogenic signaling. Upon insulin binding, it phosphorylates substrates like IRS1-4, SHC, GAB1, and CBL, initiating the PI3K-AKT and Ras-MAPK pathways.
• The PI3K-AKT pathway is primarily responsible for metabolic actions, such as GLUT4 translocation to the membrane for glucose uptake, while the Ras-MAPK pathway regulates gene expression, cell growth, and differentiation.
• Two major isoforms exist (Long and Short) via alternative splicing, and the receptor can form hybrid receptors with IGF1R, influencing ligand binding affinities. Related references: PMID:12138094 PMID:16831875
• Subcellularly, INSR localizes to the cell membrane, and upon activation undergoes internalization into late endosomes and lysosomes.
• Post-translational modifications such as phosphorylation, disulfide bond formation, glycosylation, and ubiquitination regulate its activity, trafficking, and stability.
• INSR is predominantly expressed in insulin target tissues (liver, adipose tissue, skeletal muscle) and is overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.

Experimental Guidance and Technical Tips

• This monoclonal antibody is generated against a recombinant fusion protein covering amino acids 28-944 of human CD220, which spans the majority of the extracellular domain; thus it likely recognizes an extracellular epitope, making it suitable for flow cytometry with live cells.
• For flow cytometry, optimize antibody titration and include appropriate isotype controls to ensure specific staining of human CD220 on the cell surface.
• Because the insulin receptor is expressed on a variety of cell types, validate expression in your target cell population using a positive control like HepG2 or IM-9 cells.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted sourcing partner, CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated monoclonal antibodies, antibody pairs, and ancillary reagents. This anti-human CD220 antibody supports research into insulin signaling, diabetes, and metabolic disorders, and CamelBio offers full support for procurement of bulk volumes and customization to meet your specific assay development needs.