PE Rabbit anti-Human CD207/Langerin mAb for FC - Q9UJ71

Core Product Specifications and Parameters

Biological Background: CD207/Langerin Function and Localization

• CD207, also known as Langerin or CLEC4K, is a C-type lectin domain family member that functions as a calcium-dependent, mannose-binding receptor. It induces the formation of Birbeck granules and acts as a potent regulator of membrane superimposition and zippering.
• Langerin binds a broad spectrum of glycans, including sulfated and mannosylated structures, keratan sulfate, and beta-glucans, highlighting its role in pathogen recognition.
• The receptor facilitates antigen uptake and is involved in routing and processing antigens for presentation to T cells, playing a central role in Langerhans cell-mediated immunity.
• CD207 serves as a major receptor on primary Langerhans cells for fungal species such as Candida, Saccharomyces, and Malassezia furfur, contributing to innate antifungal defense.
• It protects against HIV-1 infection by binding high-mannose structures on the viral envelope glycoprotein, subsequently targeting the virus to Birbeck granules for rapid degradation.
• Langerin is exclusively expressed by Langerhans cells in normal tissues; aberrant expression has been reported in astrocytoma and malignant ependymoma.
• The protein is a membrane-anchored glycoprotein with a single transmembrane helix, a coiled-coil domain, and intramolecular disulfide bonds, features essential for its proper folding and function.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 65-328) of human Langerin, suggesting the antibody recognizes native surface epitopes suitable for live-cell flow cytometry.
• As a PE-conjugated monoclonal antibody, it is ready for direct flow cytometric analysis; consider titrating to determine the optimal concentration for your sample system.
• Validate specificity in human Langerhans cells or CD207-transfected cell lines; cross-reactivity is limited to human, so use appropriate human tissue or cell controls.
• Include an appropriate isotype control and Fc receptor blocking step to minimize nonspecific binding in flow cytometry experiments.
• For analysis of Langerin-mediated antigen internalization, note that the antibody may activate receptor-mediated endocytosis; confirm surface staining stability under relevant experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this anti-CD207/Langerin monoclonal antibody to support dendritic cell and immunological research. We supply a wide range of validated antibodies, including fluorophore conjugates, recombinant proteins, and ancillary reagents, ensuring consistent quality and batch-to-batch reliability. Whether you need bulk quantities or rare-target custom sourcing, our streamlined supply chain and technical expertise help accelerate your development from concept to clinic.