PE Rabbit anti-Human CD205/DEC-205 Monoclonal Antibody for FC - O60449

Core Product Specifications and Parameters

Biological Background: Lymphocyte antigen 75 Function and Localization

• Encoded by the LY75 gene, lymphocyte antigen 75 (also known as DEC-205, CD205, CLEC13B, gp200-MR6) is a type I transmembrane protein belonging to the C-type lectin domain family. It exists in multiple isoforms due to alternative splicing, with isoform 2 and 3 being expressed in Hodgkin and Reed-Sternberg cells.
• Acts as an endocytic receptor that mediates the uptake of extracellular antigens and their delivery to a specialized antigen-processing compartment, thereby promoting antigen presentation and subsequent T cell activation. It also causes reduced proliferation of B-lymphocytes.
• Belongs to the C-type lectin domain family 13, characterized by a series of C-type lectin-like domains, which are involved in carbohydrate recognition and pathogen binding.
• Subcellular localization is primarily at the plasma membrane, consistent with its role as a cell-surface receptor.
• Post-translational modifications include glycosylation and phosphorylation. The protein contains disulfide bonds and a signal peptide, and is annotated as a glycoprotein and phosphoprotein.
• Exhibits tissue-specific expression in spleen, thymus, colon, and peripheral blood lymphocytes. Detected in myeloid and B-lymphoid cell lines, with isoform 2 and 3 specifically found in malignant Hodgkin lymphoma cells (Hodgkin and Reed-Sternberg cells).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 216–501, corresponding to an extracellular region rich in C-type lectin domains. For applications requiring native conformation, consider verifying reactivity with the endogenous protein on live cells.
• This antibody is directly conjugated to PE and optimized for flow cytometry. Ensure appropriate compensation and isotype controls are used, especially when analyzing heterogeneous lymphocyte populations.
• When working with human samples, validate the antibody in the relevant cell types known to express CD205, such as dendritic cells or B cells, and note that isoform-specific detection may differ in Hodgkin lymphoma.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support diagnostic development and biomedical research. This PE-conjugated anti-CD205/DEC-205 antibody, targeting a key endocytic receptor in dendritic cells and B-lymphocytes, is part of CamelBio’s immunology portfolio, and we also provide sourcing assistance for rare target reagents across the entire diagnostic workflow.