PE Rabbit anti-Human CD200R Monoclonal Antibody for Flow Cytometry - Q8TD46

Core Product Specifications and Parameters

Biological Background: CD200R Function and Localization

• CD200R1 (also known as OX2R, MOX2R) is a cell surface glycoprotein that serves as an inhibitory receptor for the CD200/OX2 ligand, playing a crucial role in immune regulation and inflammation control.
• Upon binding CD200, CD200R1 limits inflammation by inhibiting expression of pro-inflammatory molecules such as TNF, interferons, and iNOS in response to selected stimuli.
• The receptor also interacts with the HHV-8 viral CD200 homolog (K14) with comparable affinity, implicating it in host-virus interactions and immune evasion.
• CD200R1 is primarily localized to the cell membrane but can also exist as a secreted isoform, suggesting both membrane-bound and soluble regulatory functions.
• It is expressed broadly in hematopoietic cells including granulocytes, monocytes, most T-cells, neutrophils, basophils, and subsets of NK, NKT, and B-cells, as well as in tissues such as bone marrow, lymph nodes, spleen, lung, liver, spinal cord, and kidney.
• Key structural features include a signal peptide, transmembrane helix, disulfide bonds, and glycosylation sites, which contribute to its stability and ligand recognition.
• Alternative splicing generates multiple isoforms, potentially modulating its regulatory roles in immune homeostasis.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody spans amino acids 29–240, corresponding to the extracellular domain of human CD200R. This design often yields antibodies capable of recognizing the native receptor on live cells, making it well-suited for flow cytometry applications.
• For successful FC staining, titrate the antibody to determine the optimal working dilution in your specific cell system, and include appropriate Fc receptor blocking steps to minimize non-specific binding.
• Given the expression of CD200R in various leukocyte subsets, confirm antibody specificity using CD200R-negative cell lines or by pre-incubating with the immunogen as a blocking control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, enabling diagnostic manufacturers and researchers to access validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-CD200R monoclonal antibody supports flow cytometry-based immunophenotyping in inflammation and immune regulation studies. Beyond standard catalog products, we specialize in sourcing rare targets and providing customized reagent solutions to streamline your assay development from concept to clinic.