PE Rabbit anti-Human CD1c Monoclonal Antibody for Flow Cytometry - P29017

Core Product Specifications and Parameters

Biological Background: CD1c Function and Localization

• CD1c (T-cell surface glycoprotein CD1c), also designated R7, CD1, CD1A, and BDCA1, is a member of the CD1 family of antigen-presenting molecules that present lipid and glycolipid antigens to T cells.
• CD1c binds both self and non-self lipid antigens and presents them to T-cell receptors on natural killer T-cells, playing a key role in adaptive immunity.
• The protein is a type I membrane glycoprotein containing an immunoglobulin-like domain and is expressed on the surface of cortical thymocytes, certain T-cell leukemias, and a variety of other tissues.
• Subcellularly, CD1c localizes to the cell membrane, endosome membrane, and lysosome, consistent with its role in lipid antigen sampling and presentation.
• Post-translational modifications include glycosylation and the formation of a critical disulfide bond, which contribute to its structural stability and immune function.
• The mature protein has a molecular weight of approximately 37.7 kDa (predicted), with an extracellular domain that mediates lipid binding and T-cell receptor engagement.

Experimental Guidance and Technical Tips

• Since the immunogen comprises the extracellular domain (amino acids 19–298), the antibody can be considered for detecting cell surface CD1c in flow cytometry.
• For optimal flow cytometric staining, titrate the antibody to determine the ideal concentration for your specific cell type and instrument setup, and include appropriate isotype controls.
• PE conjugation offers bright fluorescence suitable for multicolor panel design; validate the antibody in the presence of other fluorophores to avoid compensation issues.
• Confirm expression of CD1c in your target cell population (e.g., dendritic cells, thymocytes) using known positive controls and consider co-staining with other lineage markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized procurement partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigen proteins, and ancillary reagents. For immunology and oncology applications targeting CD1c, we supply high-quality monoclonal antibodies and can facilitate sourcing of rare targets. Contact us to explore our complete range of one-stop solutions, from concept to clinic.