PE Rabbit anti-Human CD1B Monoclonal Antibody for Flow Cytometry - P29016

Core Product Specifications and Parameters

Biological Background: T-cell surface glycoprotein CD1b Function and Localization

• T-cell surface glycoprotein CD1b is a member of the CD1 family of antigen-presenting molecules, involved in presenting lipid and glycolipid antigens to T-cell receptors on natural killer T-cells.
• The protein undergoes alternative splicing, producing isoforms that may vary in expression and function.
• CD1b is localized to the cell membrane, as well as endosomal and lysosomal membranes, facilitating antigen binding and presentation in these compartments.
• Keywords highlight key features: glycoprotein with disulfide bonds, immunoglobulin-like domain, and transmembrane helix, important for its structural integrity and trafficking.
• Tissue expression includes cortical thymocytes, certain T-cell leukemias, and various other tissues, indicating a role in both development and disease.

Experimental Guidance and Technical Tips

• This antibody targets the extracellular domain (aa 18-303) of CD1b, and the PE conjugation allows direct flow cytometry detection without secondary reagents.
• For flow cytometry, titrate the antibody using relevant cell lines (e.g., MOLT-4 T-cell leukemia line) or primary immune cells to determine optimal concentration.
• Use an appropriate isotype control (e.g., PE-conjugated rabbit IgG monoclonal) to set gates and discriminate specific binding.
• Validate antibody performance in relevant sample systems; since CD1b is expressed on immune cells, consider testing with human PBMCs or thymocyte preparations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for sourcing IVD raw materials, providing a comprehensive portfolio that includes validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. This CD1b antibody supports research into lipid antigen presentation and T-cell immunity, areas where CamelBio’s supply chain ensures consistent access to high-quality raw materials for diagnostic and life science applications. From rare targets to large-volume needs, we bridge the gap at every stage of development.