PE Rabbit anti-Human CD197/CCR7 Monoclonal Antibody for Flow Cytometry - P32248

Core Product Specifications and Parameters

Biological Background: CD197/CCR7 Function and Localization

• CD197/CCR7 is a C-C chemokine receptor type 7, also known as BLR2, CDw197, EBI1 (Epstein-Barr virus-induced G protein-coupled receptor 1), and MIP-3 beta receptor.
• Functions as the receptor for the chemokine MIP-3-beta (CCL19), mediating lymphocyte chemotaxis and homing to secondary lymphoid organs.
• Implicated in Epstein-Barr virus (EBV) effects on B-lymphocytes; expression is strongly upregulated in EBV-infected B-cells and herpesvirus-infected T-cells.
• Belongs to the G protein-coupled receptor (GPCR) family, featuring seven transmembrane domains and a disulfide bond typical of chemokine receptors.
• Localized at the cell membrane, consistent with its role as a transmembrane receptor and signal transducer.
• Undergoes glycosylation, which may influence receptor stability, ligand binding, or trafficking.
• Expressed in various lymphoid tissues and activated B- and T-lymphocytes, highlighting its role in adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 25-59), making this antibody suitable for surface staining in flow cytometry.
• PE conjugation allows direct detection without secondary antibodies; titrate the antibody to establish optimal signal-to-noise ratio for your cell type.
• As CCR7 is expressed on activated lymphocytes, include appropriate resting and activated lymphocyte controls.
• For fixation/permeabilization, test compatibility as epitope may be sensitive.
• Since the predicted molecular weight is 43 kDa, western blot may be possible but was not validated; if attempting, use reducing conditions and include positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. Our CCR7 antibody portfolio includes validated clones for flow cytometry and other immunoassays, plus bulk ancillary reagents and custom conjugation services. Whether you need rare target antibodies, optimized pairs, or technical consultation, CamelBio streamlines procurement to accelerate your assay development.