PE Rabbit Anti-Human CD185/CXCR5 Monoclonal Antibody for Flow Cytometry - P32302

Core Product Specifications and Parameters

Biological Background: CXCR5 Function and Localization

• CXCR5 is a C-X-C chemokine receptor that specifically binds B-lymphocyte chemoattractant (BLC), orchestrating B-cell migration into B-cell follicles of spleen and Peyer patches.
• Beyond chemotaxis, it may play a regulatory role in Burkitt lymphoma (BL) lymphomagenesis and B-cell differentiation, highlighting its importance in both normal B-cell biology and lymphomagenesis.
• The protein is also known as Burkitt lymphoma receptor 1 (BLR1), monocyte-derived receptor 15 (MDR-15), and CD185, reflecting its discovery context.
• As a G protein-coupled receptor (GPCR), CXCR5 contains seven-transmembrane domains and transduces extracellular signals across the cell membrane.
• Subcellularly, CXCR5 localizes predominantly to the cell membrane, consistent with its function as a surface receptor.
• It is primarily expressed in mature B-cells and Burkitt lymphoma cells, as reported in tissue specificity studies.
• Post-translational modifications include glycosylation and disulfide bond formation, which are critical for proper folding and receptor function.
• Alternative splicing generates isoforms, possibly accounting for the observed protein weights of approximately 36 and 42 kDa.

Experimental Guidance and Technical Tips

• The immunogen used comprises the N-terminal extracellular domain (amino acids 1–47) of human CXCR5. Targeting this region may yield antibodies that recognize the native receptor on live cells, making it suitable for flow cytometry applications.
• For flow cytometry, titrate the antibody to determine the optimal working concentration for your specific sample type (e.g., PBMCs, cultured B-cell lines).
• Include appropriate isotype controls and Fc-receptor blocking steps to minimize nonspecific binding.
• Since CXCR5 is expressed on mature B-cells, consider validating expression on known positive and negative cell populations to confirm specificity.
• The antibody is supplied as a PE conjugate, suitable for direct staining without need for a secondary detection step.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focusing on B-cell biology, chemokine receptors, or lymphoma research, this anti-CD185/CXCR5 monoclonal antibody is a reliable tool for flow cytometry assays. CamelBio streamlines sourcing with validated antibody pairs, optimized bulk monoclonal/polyclonal antibodies, ancillary reagents, and rare-target raw material procurement to support your diagnostic and translational development needs.