PE Rabbit anti-Human CD172a/SIRPα Monoclonal Antibody for Flow Cytometry - P78324

Core Product Specifications and Parameters

Biological Background: CD172a/SIRPα Function and Localization

• Encoded by the SIRPA gene, this protein is a tyrosine-protein phosphatase non-receptor type substrate 1, also known as SIRPα, CD172a, and SHPS1. It belongs to the signal-regulatory protein family and exists as multiple isoforms (Sirp-alpha-1/2/3) due to alternative splicing.
• Functions as an immunoglobulin-like cell surface receptor for CD47, acting as a docking protein that recruits and induces translocation of phosphatases PTPN6 and PTPN11 from the cytosol to the plasma membrane.
• Negatively regulates phagocytosis, mast cell activation, and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
• Supports adhesion of cerebellar neurons, promotes neurite outgrowth, and facilitates glial cell attachment, indicating a role in synaptogenesis and synaptic function (By similarity).
• Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors, or insulin.
• Serves as a receptor for thrombospondin-1 (THBS1), leading to phosphorylation of SIRPα and subsequent ROS production through NADPH oxidase in non-phagocytic cells. Related references: PMID:24511121
• Subcellular localization is at the membrane, consistent with its role as a transmembrane receptor. Post-translational modifications include phosphorylation and glycosylation.
• Ubiquitously expressed, with highest levels in brain; also detected in heart, placenta, lung, liver, and various other tissues, but not on T-cells.

Experimental Guidance and Technical Tips

• As this antibody is PE-conjugated, it is suitable for direct flow cytometry without the need for a secondary antibody.
• The immunogen corresponds to the extracellular domain (amino acids 31-370), suggesting the antibody targets an extracellular epitope; thus, live-cell staining protocols may be applicable.
• For optimal performance, consider titrating the antibody to determine the best concentration for your specific cell type and experiment.
• Validate the antibody in the relevant sample system, such as human myeloid cells or brain tissue, given the target's expression profile.

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• CamelBio bridges the gap from concept to clinic by providing one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-CD172a/SIRPα antibody for immunology and neuroscience research.
• In addition to ready-to-use conjugated antibodies, we supply matched antibody pairs, recombinant proteins, and bulk ancillary reagents to support your diagnostic or research workflows.
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