PE Rabbit anti-Human CD167a/DDR1 Monoclonal Antibody for FC - Q08345

Core Product Specifications and Parameters

Biological Background: CD167a/DDR1 Function and Localization

• CD167a/DDR1, also known as epithelial discoidin domain-containing receptor 1, is a tyrosine kinase receptor encoded by the DDR1 gene, with multiple aliases including CAK, EDDR1, MCK10, NTRK4, PTK3A, RTK6, and TRKE.
• DDR1 acts as a cell surface receptor for fibrillar collagen, and upon ligand binding, triggers downstream signaling involving SRC and MAP kinases, regulating cell attachment, migration, survival, and proliferation.
• It plays a key role in extracellular matrix remodeling by upregulating matrix metalloproteinases (MMP2, MMP7, MMP9), thereby facilitating cell migration and wound healing.
• DDR1 is required for normal developmental processes, including blastocyst implantation during pregnancy, mammary gland differentiation and lactation, and normal ear morphology and hearing.
• In arterial smooth muscle cells, DDR1 promotes migration and contributes to wound healing; it also has a role in tumor cell invasion.
• The receptor is predominantly localized at the cell membrane, with a secreted isoform also reported; it is expressed at the highest levels in brain, lung, placenta, and kidney, and is abundant in various carcinoma cell lines.
• DDR1 undergoes alternative splicing, is glycosylated, and contains ATP-binding and kinase domains; post-translational modifications include phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 21-416 of human CD167a/DDR1, which includes the extracellular domain; this design may help target the native conformation of the receptor.
• This antibody is validated for flow cytometry (FC); consider titrating the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• For FC experiments, include appropriate isotype controls and ensure proper gating to exclude dead cells and debris; CD167a/DDR1 expression may vary between cell lines and tissues, so validate in the relevant sample system.
• If using this antibody in other applications not listed, perform orthogonal validation to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this PE-conjugated anti-CD167a/DDR1 antibody for flow cytometry applications.
• Whether you need bulk ancillary reagents, optimized antibody pairs, or assistance sourcing rare-target raw materials, we provide end-to-end support from concept to clinic.
• Our expertise in molecular biology reagents ensures reliable supply for cancer research, cell signaling studies, and diagnostic development focused on receptor tyrosine kinases.