PE Rabbit anti-Human CD166/ALCAM Monoclonal Antibody for Flow Cytometry - Q13740

Core Product Specifications and Parameters

Biological Background: CD166/ALCAM Function and Localization

• CD166 (ALCAM, MEMD) is a 65 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily; it also exists as a secreted isoform that antagonizes membrane-bound functions.
• Promotes heterotypic cell adhesion by binding to CD6, thereby activating T-cell proliferation and cytokine production. Related references: PMID:15048703, PMID:16352806, PMID:24945728
• Engages CD6 to contribute to the formation and maturation of the immunological synapse. Related references: PMID:15294938, PMID:16352806
• Mediates homotypic ALCAM-ALCAM interactions, facilitating dendritic cell attachment to endothelia, inhibiting endothelial migration, and promoting tube formation. Related references: PMID:15496415, PMID:23169771
• Serves as a ligand for LILRB4, augmenting its inhibitory effect on T-cell proliferation. Related references: PMID:29263213
• Essential for hematopoietic stem cell engraftment in bone marrow. Related references: PMID:24740813
• Located at the cell membrane, axonal and dendritic projections; the protein is N-glycosylated and stabilized by disulfide bonds.
• Expressed on hematopoietic stem cells, activated T/B lymphocytes, monocytes, dendritic cells, endothelial cells, neurons, and various tumor lines; high expression correlates with melanoma metastasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full extracellular domain (aa 28–526), ensuring recognition of surface-exposed epitopes in live-cell flow cytometry.
• PE conjugation provides bright signal with low background; titrate antibody to determine optimal staining concentration for the target cell population.
• Validate staining specificity using ALCAM-knockout or blocking peptides; include appropriate isotype controls.
• For multicolor panels, ensure compensation using single-stained samples; PE is compatible with common laser/filter setups (e.g., 488 nm laser, 575/26 BP filter).
• Consider gentle cell handling to preserve surface antigen integrity; avoid over-fixation or over-permeabilization that may alter epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a strategic one-stop partner for IVD raw-material procurement, bridging diagnostic manufacturers and research institutions with rigorously validated reagents like this anti-CD166 monoclonal antibody. Covering immunology, oncology, and stem cell applications, our portfolio spans validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Scientists developing flow cytometry-based assays can source rare-target raw materials and receive consultative guidance from concept to clinical application through CamelBio’s integrated supply ecosystem.