PE Rabbit anti-Human CD161 mAb for Flow Cytometry - Q12918

Core Product Specifications and Parameters

Biological Background: CD161 (KLRB1) Function and Localization

• KLRB1 encodes Killer cell lectin-like receptor subfamily B member 1, also known as CD161, NKR-P1A, and CLEC5B.
• CD161 plays an inhibitory role on natural killer (NK) cell cytotoxicity. Activation stimulates acid sphingomyelinase/SMPD1, elevates intracellular ceramide, and enhances T-cell proliferation.
• It acts as a lectin that binds to the terminal Gal-alpha(1,3)Gal epitope and N-acetyllactosamine. CD161 also binds to its ligand CLEC2D/LLT1, inhibiting NK cell-mediated cytotoxicity and interferon-gamma secretion in target cells.
• CD161 is a membrane protein with a signal-anchor and transmembrane helix, classified under the C-type lectin domain family.
• Expressed in a subset of NK cells, predominantly in intestinal epithelium and liver, and detected in peripheral blood T-cells, preferably with a memory antigenic phenotype.
• Post-translational modifications include glycosylation and disulfide bond formation.
• The protein has a calculated molecular weight of approximately 25 kDa.

Experimental Guidance and Technical Tips

• For flow cytometry, consider titrating the antibody to determine the optimal working concentration for your specific experimental setup.
• Validate staining using relevant human NK cell lines or peripheral blood mononuclear cells (PBMCs). An appropriate isotype control is recommended.
• Due to the PE conjugate, avoid fluorochrome combinations that may cause spectral overlap; ensure proper compensation.
• The immunogen spans the extracellular C-type lectin domain; confirm surface staining under non-permeabilizing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. This PE anti-human CD161 antibody is part of our immunology portfolio; we can supply matched antibody pairs, bulk ancillary reagents, and custom rare-target sourcing to streamline your NK cell research and assay development. Contact us to discuss your specific procurement needs.