PE Rabbit anti-Human CD158a/KIR2DL1 Monoclonal Antibody for Flow Cytometry - P43626

Core Product Specifications and Parameters

Biological Background: CD158a/KIR2DL1 Function and Localization

• KIR2DL1, also known as CD158a, NKAT1, or p58.1, is a killer cell immunoglobulin-like receptor (KIR) belonging to the immunoglobulin superfamily, characterized by extracellular immunoglobulin domains and a transmembrane helix.
• It functions as an inhibitory receptor on natural killer (NK) cells, specifically recognizing certain HLA-C alleles (e.g., w4 and w6), and suppresses NK cell-mediated cytotoxicity to prevent target cell lysis.
• The receptor is a transmembrane glycoprotein with disulfide bond-stabilized immunoglobulin domains, a signal peptide, and a cytoplasmic tail that transduces inhibitory signals.
• KIR2DL1 is primarily expressed on the surface of NK cells, playing a critical role in immune surveillance and self-tolerance by monitoring MHC class I molecule expression.
• Alternative splicing generates multiple isoforms of the receptor, and post-translational modifications include N-linked glycosylation and disulfide bond formation, which are essential for proper folding and function.
• Subcellularly, KIR2DL1 localizes to the cell membrane, where its extracellular region engages ligands on target cells, while its intracellular domain recruits phosphatases to inhibit activation signals.
• The three-dimensional structure of KIR2DL1, resolved through crystallography, reveals key ligand-binding interfaces and conformational changes upon HLA-C interaction.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions, as staining intensity may vary across NK cell subsets.
• Since the immunogen encompasses the extracellular domain (amino acids 22–245), this antibody is suitable for surface staining of live, unfixed cells without the need for permeabilization.
• Validate the antibody on well-characterized NK cell lines or primary human NK cells to confirm specificity, as KIR2DL1 expression is clonally distributed and activation-dependent.
• Use appropriate isotype controls and, if analyzing multiple KIR family members, consider multi-color panels with carefully selected fluorochromes to minimize compensation artifacts.
• When applied in functional assays, note that antibody binding may block ligand recognition or mimic receptor engagement; pre-test in your system to assess potential agonist/antagonist effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive partner for diagnostic manufacturers and research laboratories, offering one-stop access to IVD raw materials, technical services, and consulting support from concept to clinic. For investigators focusing on NK cell immunology and immune checkpoint research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Leveraging our extensive network, we also facilitate sourcing of rare-target raw materials, ensuring secure and efficient supply chains for your projects.